EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.2 | - |
Comamonas testosteroni |
1.14.13.23 | 1.4-1.6 M ammonium sulfate, 4-8% dioxane, pH 6.5 | Comamonas testosteroni |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | iodoacetamide | reversed by dithiothreitol | Comamonas testosteroni | |
1.14.13.2 | N-iodosuccinimide | reversed by dithiothreitol | Comamonas testosteroni | |
1.14.13.2 | o-Iodosobenzoate | reversed by dithiothreitol | Comamonas testosteroni | |
1.14.13.2 | p-hydroxy-3-iodomethylbenzoate | 1 mM, irreversible crosslinking to the substrate binding site | Comamonas testosteroni | |
1.14.13.23 | 4-hydroxy-3-iodomethylbenzoate | inhibition not reversed in presence of dithiotreitol | Comamonas testosteroni | |
1.14.13.23 | iodoacetamide | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni | |
1.14.13.23 | N-iodosuccinimide | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni | |
1.14.13.23 | o-Iodosobenzoate | inhibition reversed in presence of dithiotreitol | Comamonas testosteroni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.2 | 71000 | - |
x * 71000, SDS-PAGE | Comamonas testosteroni |
1.14.13.23 | 71000 | - |
x * 71000, SDS-PAGE | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | Comamonas testosteroni | - |
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.2 | Comamonas testosteroni | - |
- |
- |
1.14.13.23 | Comamonas testosteroni | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.2 | - |
Comamonas testosteroni |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.23 | 6.03 | - |
- |
Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Comamonas testosteroni | protocatechuate + NADP+ + H2O | - |
? | |
1.14.13.23 | 3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.13.23 | gentisate + NADPH + O2 | - |
Comamonas testosteroni | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.2 | ? | x * 71000, SDS-PAGE | Comamonas testosteroni |
1.14.13.23 | ? | x * 71000, SDS-PAGE | Comamonas testosteroni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.23 | 6.2 | - |
in potassium phosphate buffer | Comamonas testosteroni |
1.14.13.23 | 7.3 | - |
in Tris-HCl buffer | Comamonas testosteroni |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.23 | FAD | flavoprotein | Comamonas testosteroni | |
1.14.13.23 | NADPH | specific requirement for NADPH | Comamonas testosteroni |