EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.13.2 | crystallization of mutant enzymes H162R and R269T by hanging drop vapour diffusion method | Pseudomonas fluorescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.2 | H162D | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | H162K | less efficient than wild-type enzyme due to a clear increase in the apparent Km-value for NADPH | Pseudomonas fluorescens |
1.14.13.2 | H162N | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | H162R | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
1.14.13.2 | H162S | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | H162T | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | H162Y | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
1.14.13.2 | R269D | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | R269K | rather efficient enzyme with similar catalytic properties as wild-type enzyme | Pseudomonas fluorescens |
1.14.13.2 | R269N | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | R269S | less efficient than wild-type enzyme due to a clear increase in the apparent Km-value for NADPH | Pseudomonas fluorescens |
1.14.13.2 | R269T | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
1.14.13.2 | R269Y | no reliable turnover rate due to impaired NADPH binding | Pseudomonas fluorescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.2 | diethyl dicarbonate | inhibition of wild-type enzyme, no inhibition of mutant enzyme H162R | Pseudomonas fluorescens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | additional information | - |
additional information | Km value for NADPH above 0.5 mM: mutant enzymes R269D, R269T, R269N, R269Y, H162D, H162T, H162S, H162N | Pseudomonas fluorescens | |
1.14.13.2 | 0.02 | - |
4-hydroxybenzoate | wild-type enzyme | Pseudomonas fluorescens | |
1.14.13.2 | 0.03 | - |
NADPH | wild-type enzyme | Pseudomonas fluorescens | |
1.14.13.2 | 0.03 | - |
4-hydroxybenzoate | mutant enzyme H162R, H162Y, H162K, R269K, R269Y, R269N, R269S and R269T | Pseudomonas fluorescens | |
1.14.13.2 | 0.04 | - |
4-hydroxybenzoate | mutant enzyme H162N, H162S, H162T and H162D | Pseudomonas fluorescens | |
1.14.13.2 | 0.05 | - |
NADPH | mutant enzyme H162R | Pseudomonas fluorescens | |
1.14.13.2 | 0.06 | - |
4-hydroxybenzoate | mutant enzyme R269D | Pseudomonas fluorescens | |
1.14.13.2 | 0.07 | - |
NADPH | mutant enzyme H162Y and R269K | Pseudomonas fluorescens | |
1.14.13.2 | 0.2 | - |
NADPH | mutant enzyme H162K | Pseudomonas fluorescens | |
1.14.13.2 | 0.32 | - |
NADPH | mutant enzyme R269S | Pseudomonas fluorescens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.2 | Pseudomonas fluorescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.2 | - |
Pseudomonas fluorescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.2 | 4-hydroxybenzoate + NADPH + O2 | - |
Pseudomonas fluorescens | protocatechuate + NADP+ + H2O | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.2 | additional information | - |
additional information | - |
Pseudomonas fluorescens |