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Literature summary extracted from

  • Jadan, A.P.; van Berkel, W.J.H.; Golovleva, L.A.; Golovlev, E.L.
    Purification and properties of p-hydroxybenzoate hydroxylases from Rhodococcus strains (2001), Biochemistry (Moscow), 66, 898-903.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.2 enzyme rapidly loses activity in dilute solutions, below 2 mg/ml, without stabilizer Rhodococcus rhodochrous
1.14.13.2 enzyme rapidly loses activity in dilute solutions, below 2 mg/ml, without stabilizer Rhodococcus rhodnii
1.14.13.2 enzyme rapidly loses activity in dilute solutions, below 2 mg/ml, without stabilizer Rhodococcus sp.
1.14.13.2 enzyme rapidly loses activity in dilute solutions, below 2 mg/ml, without stabilizer Rhodococcus opacus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.003
-
 4-hydroxybenzoate strain 557 Rhodococcus opacus
1.14.13.2 0.0033
-
 4-hydroxybenzoate
-
 Rhodococcus sp.
1.14.13.2 0.0072
-
 4-hydroxybenzoate
-
 Rhodococcus rhodochrous
1.14.13.2 0.0073
-
 4-hydroxybenzoate
-
 Rhodococcus rhodnii
1.14.13.2 0.0079
-
 4-hydroxybenzoate strain IG Rhodococcus opacus
1.14.13.2 0.0081
-
 4-hydroxybenzoate strain 420 Rhodococcus opacus
1.14.13.2 0.0122
-
 NADH strain 557 Rhodococcus opacus
1.14.13.2 0.0139
-
 NADH
-
 Rhodococcus sp.
1.14.13.2 0.0167
-
 NADH
-
 Rhodococcus rhodochrous
1.14.13.2 0.0195
-
 NADH strain IG Rhodococcus opacus
1.14.13.2 0.02
-
 NADH strain 420 Rhodococcus opacus
1.14.13.2 0.0226
-
 NADPH
-
 Rhodococcus rhodochrous
1.14.13.2 0.0238
-
 NADPH strain IG Rhodococcus opacus
1.14.13.2 0.0335
-
 NADPH strain 557 Rhodococcus opacus
1.14.13.2 0.0398
-
 NADH
-
 Rhodococcus rhodnii
1.14.13.2 0.0658
-
 NADPH strain 420 Rhodococcus opacus
1.14.13.2 0.155
-
 NADPH
-
 Rhodococcus rhodnii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 45000
-
 4 * 45000, SDS-PAGE Rhodococcus rhodochrous
1.14.13.2 45000
-
 4 * 45000, SDS-PAGE Rhodococcus sp.
1.14.13.2 46000
-
 4 * 46000, strain 557, SDS-PAGE Rhodococcus opacus
1.14.13.2 47500
-
 4 * 47500, strain 420, SDS-PAGE Rhodococcus opacus
1.14.13.2 48500
-
 4 * 48500, SDS-PAGE Rhodococcus rhodnii
1.14.13.2 51000
-
 4 * 51000, strain IG, SDS-PAGE Rhodococcus opacus

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Rhodococcus opacus
-
 557, 420, and 1G
-
1.14.13.2 Rhodococcus rhodnii
-
 135
-
1.14.13.2 Rhodococcus rhodnii 135
-
 135
-
1.14.13.2 Rhodococcus rhodochrous
-
 172
-
1.14.13.2 Rhodococcus rhodochrous 172
-
 172
-
1.14.13.2 Rhodococcus sp.
-
 400
-
1.14.13.2 Rhodococcus sp. 400
-
 400
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.2
-
 Rhodococcus rhodnii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.2 25
-
-
 Rhodococcus rhodnii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus rhodochrous protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus rhodnii protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus sp. protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus opacus protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus rhodnii 135 protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus rhodochrous 172 protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADH + O2
-
 Rhodococcus sp. 400 protocatechuate + NAD+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus rhodochrous protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus rhodnii protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus sp. protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus opacus protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus rhodnii 135 protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus rhodochrous 172 protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Rhodococcus sp. 400 protocatechuate + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.2 tetramer 4 * 45000, SDS-PAGE Rhodococcus rhodochrous
1.14.13.2 tetramer 4 * 45000, SDS-PAGE Rhodococcus sp.
1.14.13.2 tetramer 4 * 48500, SDS-PAGE Rhodococcus rhodnii
1.14.13.2 tetramer 4 * 46000, strain 557, SDS-PAGE Rhodococcus opacus
1.14.13.2 tetramer 4 * 47500, strain 420, SDS-PAGE Rhodococcus opacus
1.14.13.2 tetramer 4 * 51000, strain IG, SDS-PAGE Rhodococcus opacus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.2 50
-
 50% loss of activity after 9 min, strain IG Rhodococcus opacus
1.14.13.2 50
-
 50% loss of activity after 180 min Rhodococcus rhodnii
1.14.13.2 50
-
 50% loss of activity after 37 min Rhodococcus sp.
1.14.13.2 50
-
 50% loss of activity after 50 min Rhodococcus rhodochrous
1.14.13.2 50
-
 50% loss of activity after 45 min, strain 557 Rhodococcus opacus
1.14.13.2 50
-
 50% loss of activity after 15 min, strain 420 Rhodococcus opacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.2 additional information
-
 additional information
-
 Rhodococcus rhodochrous
1.14.13.2 additional information
-
 additional information
-
 Rhodococcus rhodnii
1.14.13.2 additional information
-
 additional information
-
 Rhodococcus sp.
1.14.13.2 additional information
-
 additional information
-
 Rhodococcus opacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.2 7
-
-
 Rhodococcus rhodochrous
1.14.13.2 7.2
-
 strain 400 and 557 Rhodococcus opacus
1.14.13.2 7.4
-
-
 Rhodococcus sp.
1.14.13.2 7.4
-
 strain IG Rhodococcus opacus
1.14.13.2 7.7
-
-
 Rhodococcus rhodnii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.2 7 7.5 optimal stability Rhodococcus sp.
1.14.13.2 9 9.5 optimal stability Rhodococcus opacus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD Km for strain 557: 190 nM Rhodococcus opacus
1.14.13.2 FAD Km: 420 nM Rhodococcus rhodochrous
1.14.13.2 FAD Km for strain 420: 220 nM Rhodococcus opacus
1.14.13.2 FAD Km: 225 nM Rhodococcus rhodnii
1.14.13.2 FAD Km: 440 nM Rhodococcus sp.
1.14.13.2 FAD Km for strain IG: 185 nM Rhodococcus opacus
1.14.13.2 NADH
-
 Rhodococcus rhodochrous
1.14.13.2 NADH
-
 Rhodococcus rhodnii
1.14.13.2 NADH
-
 Rhodococcus sp.
1.14.13.2 NADH
-
 Rhodococcus opacus
1.14.13.2 NADPH
-
 Rhodococcus rhodochrous
1.14.13.2 NADPH
-
 Rhodococcus rhodnii
1.14.13.2 NADPH
-
 Rhodococcus sp.
1.14.13.2 NADPH
-
 Rhodococcus opacus