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Literature summary extracted from

  • Husain, M.; Schopfer, L.M.; Massey, V.
    p-Hydroxybenzoate hydroxylase and melilotate hydroxylase (1978), Methods Enzymol., 53, 543-558.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.2
-
Pseudomonas fluorescens

General Stability

EC Number General Stability Organism
1.14.13.2 extremely unstable, undergoing rapid inactivation unless protected by substrates and other stabilizing agents Pseudomonas putida
1.14.13.2 stable to freezing and thawing Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.2 4-hydroxybenzoate mixed-type Pseudomonas fluorescens
1.14.13.2 Cl-
-
Delftia acidovorans
1.14.13.2 Cl-
-
Pseudomonas fluorescens
1.14.13.2 I-
-
Delftia acidovorans
1.14.13.2 I-
-
Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 65000
-
gel filtration Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Delftia acidovorans
-
-
-
1.14.13.2 Pseudomonas fluorescens
-
-
-
1.14.13.2 Pseudomonas putida
-
M-6
-
1.14.13.2 Pseudomonas putida
-
A 3.12
-
1.14.13.2 Pseudomonas putida A 3.12
-
A 3.12
-
1.14.13.2 Pseudomonas putida M-6
-
M-6
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.2
-
Pseudomonas fluorescens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.13.2 4-hydroxybenzoate + NADPH + H+ + O2 = 3,4-dihydroxybenzoate + NADP+ + H2O bi uni uni uni ping-pong mechanism Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.2 47.5
-
-
Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
1.14.13.2 4°C, as ammonium sulfate paste, indefinitely stable Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 2,4-dihydroxybenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
?
1.14.13.2 3-Fluoro-4-hydroxybenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
1.14.13.2 4-aminobenzoate + NADPH + O2 about 1% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Delftia acidovorans protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida A 3.12 protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
Pseudomonas putida M-6 protocatechuate + NADP+ + H2O
-
?
1.14.13.2 4-mercaptobenzoate + NADPH + O2 50% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 4,4'-dithiobisbenzoate + ?
-
?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.2 25
-
50 h, stable Pseudomonas fluorescens
1.14.13.2 25
-
pH 6.4-8.0, 50 h, stable Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD contains 1 mol of FAD per mol of enzyme Pseudomonas fluorescens
1.14.13.2 NADPH
-
Pseudomonas putida
1.14.13.2 NADPH
-
Delftia acidovorans
1.14.13.2 NADPH no activity with NADH Pseudomonas fluorescens