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Literature summary extracted from

  • Spector, T.; Massey, V.
    p-Hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Evidence for an oxygenated flavin intermediate (1972), J. Biol. Chem., 247, 5632-5636.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.014
-
 2,4-Dihydroxybenzoate
-
 Pseudomonas fluorescens
1.14.13.2 0.027
-
 NADP+ reaction with 2,4-dihydroxybenzoate Pseudomonas fluorescens
1.14.13.2 0.05
-
 O2 reaction with 2,4-dihydroxybenzoate Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 2,4-dihydroxybenzoate + NADPH + O2 slow reaction, formation of at least 3 intermediates, a spectral intermediate that is believed to be an oxygenated form of the enzyme-bound flavin prosthetic group Pseudomonas fluorescens 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 NADPH
-
 Pseudomonas fluorescens