Literature summary extracted from

Spector, T.; Massey, V.
Studies on the effector specificity of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens (1972), J. Biol. Chem., 247, 4679-4687.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.14.13.2	2,4-Dihydroxybenzoate	increases the rate of NADP oxidation by 4-hydroxybenzoate, no hydroxylation during subsequent reoxidation by O2	Pseudomonas fluorescens
1.14.13.2	3,4-dihydroxybenzoate	increases the rate of NADP oxidation by 4-hydroxybenzoate, no hydroxylation during subsequent reoxidation by O2	Pseudomonas fluorescens
1.14.13.2	benzoate	increases the rate of NADP oxidation by 4-hydroxybenzoate, no hydroxylation during subsequent reoxidation by O2	Pseudomonas fluorescens

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.14.13.2	3,4-dihydroxybenzoate	at high concentration	Pseudomonas fluorescens
1.14.13.2	4-Aminobenzoate	-	Pseudomonas fluorescens
1.14.13.2	4-Fluorobenzoate	slight	Pseudomonas fluorescens
1.14.13.2	4-hydroxybenzaldehyde	slight	Pseudomonas fluorescens
1.14.13.2	4-hydroxybenzoate	at high concentrations	Pseudomonas fluorescens
1.14.13.2	4-hydroxycinnamate	-	Pseudomonas fluorescens
1.14.13.2	6-Hydroxynicotinate	at high concentrations	Pseudomonas fluorescens
1.14.13.2	benzoate	slight	Pseudomonas fluorescens
1.14.13.2	salicylate	slight	Pseudomonas fluorescens

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.13.2	Pseudomonas fluorescens	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.13.2	2,4-dihydroxybenzoate + NADPH + O2	-	Pseudomonas fluorescens	2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O	-	?
1.14.13.2	4-hydroxybenzoate + NADPH + O2	-	Pseudomonas fluorescens	protocatechuate + NADP+ + H2O	-	?

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.13.2	NADPH	-	Pseudomonas fluorescens

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Release 2023.1 (January 2023)