EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.4 | 2-mercaptoethanol | stimulated by 2 mM | Pseudomonas putida | |
1.13.11.4 | 2-mercaptoethanol | stimulated by 2 mM | Pseudomonas alcaligenes | |
1.13.11.4 | Fe2+ | - |
Pseudomonas putida | |
1.13.11.4 | Fe2+ | - |
Pseudomonas alcaligenes | |
1.13.11.4 | glycerol | stimulated by 10% (v/v) | Pseudomonas putida | |
1.13.11.4 | glycerol | stimulated by 10% (v/v) | Pseudomonas alcaligenes | |
1.13.11.4 | L-cysteine | stimulated by 2 mM | Pseudomonas putida | |
1.13.11.4 | L-cysteine | stimulated by 2 mM | Pseudomonas alcaligenes |
EC Number | General Stability | Organism |
---|---|---|
1.13.11.4 | highly unstable in 50 mM MOPS buffer with no additives | Pseudomonas putida |
1.13.11.4 | highly unstable in 50 mM MOPS buffer with no additives | Pseudomonas alcaligenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.4 | Cu2+ | 5 mM complete inactivation; complete inhibition at 5 mM | Pseudomonas alcaligenes | |
1.13.11.4 | Cu2+ | 5 mM complete inactivation; complete inhibition at 5 mM | Pseudomonas putida | |
1.13.11.4 | EDTA | 60.24% residual activity at 5 mM | Pseudomonas alcaligenes | |
1.13.11.4 | EDTA | 4.95% residual activity at 5 mM | Pseudomonas putida | |
1.13.11.4 | Mg2+ | 66% residual activity at 5 mM | Pseudomonas putida | |
1.13.11.4 | Mn2+ | 73.96% residual activity at 5 mM | Pseudomonas alcaligenes | |
1.13.11.4 | Mn2+ | 22.36% residual activity at 5 mM | Pseudomonas putida | |
1.13.11.4 | additional information | K+, Na+, Mg2+, and Ca2+ do not inhibit the activity | Pseudomonas alcaligenes | |
1.13.11.4 | additional information | K+, Na+, and Ca2+, do not inhibit the activity | Pseudomonas putida | |
1.13.11.4 | Zn2+ | 2.58% residual activity at 5 mM | Pseudomonas alcaligenes | |
1.13.11.4 | Zn2+ | 4.69% residual activity at 5 mM | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.4 | 0.0043 | - |
3-Isopropylgentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas putida | |
1.13.11.4 | 0.011 | - |
3-Bromogentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas putida | |
1.13.11.4 | 0.015 | - |
3-Bromogentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas alcaligenes | |
1.13.11.4 | 0.036 | - |
3-Methylgentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas putida | |
1.13.11.4 | 0.053 | - |
3-Isopropylgentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas alcaligenes | |
1.13.11.4 | 0.076 | - |
3-Methylgentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas alcaligenes | |
1.13.11.4 | 0.092 | - |
gentisate | - |
Pseudomonas alcaligenes | |
1.13.11.4 | 0.092 | - |
gentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas alcaligenes | |
1.13.11.4 | 0.143 | - |
gentisate | - |
Pseudomonas putida | |
1.13.11.4 | 0.143 | - |
gentisate | in 0.1 M phosphate buffer, pH 7.4, at 23°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.4 | Fe2+ | strongly stimulated by 0.1 mM Fe2+ | Pseudomonas putida | |
1.13.11.4 | Fe2+ | strongly stimulated by 0.1 mM Fe2+ | Pseudomonas alcaligenes | |
1.13.11.4 | additional information | K+, Na+, and Ca2+, do not stimulate the activity | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 39000 | - |
4 * 39000, SDS-PAGE | Pseudomonas alcaligenes |
1.13.11.4 | 41000 | - |
2 * 41000 | Pseudomonas putida |
1.13.11.4 | 41000 | - |
2 * 41000, gel filtration | Pseudomonas putida |
1.13.11.4 | 82000 | - |
gel filtration | Pseudomonas putida |
1.13.11.4 | 154000 | - |
gel filtration | Pseudomonas alcaligenes |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.4 | Pseudomonas alcaligenes | - |
- |
- |
1.13.11.4 | Pseudomonas alcaligenes NCIB 9867 | - |
- |
- |
1.13.11.4 | Pseudomonas putida | - |
- |
- |
1.13.11.4 | Pseudomonas putida NCIB 9869 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.4 | - |
Pseudomonas putida |
1.13.11.4 | - |
Pseudomonas alcaligenes |
1.13.11.4 | ammonium sulfate fractionation, DEAE-Sepharose fast flow column chromatography | Pseudomonas putida |
1.13.11.4 | ammonium sulfate fractionation, DEAE-Sepharose fast flow column chromatography | Pseudomonas alcaligenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 0.45 | - |
crude extract, at 23°C | Pseudomonas alcaligenes |
1.13.11.4 | 0.72 | - |
crude extract, at 23°C | Pseudomonas putida |
1.13.11.4 | 23.2 | - |
after 51.56fold purification, at 23°C | Pseudomonas alcaligenes |
1.13.11.4 | 43.14 | - |
after 59.92fold purification, at 23°C | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 3.5% residual activity | Pseudomonas putida |
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 12 months, 42.9% residual activity | Pseudomonas alcaligenes |
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 42.5% residual activity | Pseudomonas putida |
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 3 months, 86.5% residual activity | Pseudomonas alcaligenes |
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 23.1% residual activity | Pseudomonas putida |
1.13.11.4 | -20°C, MOPS buffer with 0.1 mM Fe2+, 2 mM L-cysteine, and 10% (v/v) glycerol, 6 months, 70.9% residual activity | Pseudomonas alcaligenes |
1.13.11.4 | 4°C, MOPS buffer with no additives, 24 h, 22.5% residual activity | Pseudomonas alcaligenes |
1.13.11.4 | 4°C, MOPS buffer with no additives, 24 h, 33.9% residual activity | Pseudomonas putida |
1.13.11.4 | 4°C, MOPS buffer with no additives, 96 h, complete loss of activity | Pseudomonas putida |
1.13.11.4 | 4°C, MOPS buffer with no additives, 96 h, complete loss of activity | Pseudomonas alcaligenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Pseudomonas putida | maleylpyruvate | - |
? | |
1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Pseudomonas alcaligenes | maleylpyruvate | - |
? | |
1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Pseudomonas alcaligenes NCIB 9867 | maleylpyruvate | - |
? | |
1.13.11.4 | 2,5-dihydroxybenzoate + O2 | - |
Pseudomonas putida NCIB 9869 | maleylpyruvate | - |
? | |
1.13.11.4 | 3-bromogentisate + O2 | - |
Pseudomonas putida | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-bromogentisate + O2 | - |
Pseudomonas alcaligenes | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-bromogentisate + O2 | - |
Pseudomonas alcaligenes NCIB 9867 | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-bromogentisate + O2 | - |
Pseudomonas putida NCIB 9869 | 3-bromo-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-isopropyl-gentisate + O2 | - |
Pseudomonas putida | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-isopropyl-gentisate + O2 | - |
Pseudomonas alcaligenes | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-isopropyl-gentisate + O2 | - |
Pseudomonas alcaligenes NCIB 9867 | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-isopropyl-gentisate + O2 | - |
Pseudomonas putida NCIB 9869 | 4-hydroxy-3-isopropyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-methylgentisate + O2 | - |
Pseudomonas putida | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-methylgentisate + O2 | - |
Pseudomonas alcaligenes | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-methylgentisate + O2 | - |
Pseudomonas alcaligenes NCIB 9867 | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | 3-methylgentisate + O2 | - |
Pseudomonas putida NCIB 9869 | 4-hydroxy-3-methyl-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
1.13.11.4 | gentisate + O2 | - |
Pseudomonas putida | maleylpyruvate | - |
? | |
1.13.11.4 | gentisate + O2 | - |
Pseudomonas alcaligenes | maleylpyruvate | - |
? | |
1.13.11.4 | gentisate + O2 | - |
Pseudomonas alcaligenes NCIB 9867 | maleylpyruvate | - |
? | |
1.13.11.4 | gentisate + O2 | - |
Pseudomonas putida NCIB 9869 | maleylpyruvate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.4 | dimer | 2 * 41000 | Pseudomonas putida |
1.13.11.4 | homodimer | 2 * 41000, gel filtration | Pseudomonas putida |
1.13.11.4 | homotetramer | 4 * about 39000, gel filtration | Pseudomonas alcaligenes |
1.13.11.4 | tetramer | 4 * 39000, SDS-PAGE | Pseudomonas alcaligenes |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.4 | P25X gentisate 1,2-dioxygenase | - |
Pseudomonas alcaligenes |
1.13.11.4 | P35X gentisate 1,2-dioxygenase | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 50 | - |
- |
Pseudomonas putida |
1.13.11.4 | 50 | - |
- |
Pseudomonas alcaligenes |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 50 | - |
retains 35% of its activity when incubated at 50°C for 5 min | Pseudomonas putida |
1.13.11.4 | 50 | - |
retains 73% of its activity when incubated at 50°C for 5 min | Pseudomonas alcaligenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 8 | - |
- |
Pseudomonas putida |
1.13.11.4 | 8 | - |
- |
Pseudomonas alcaligenes |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.4 | 5 | 7.5 | - |
Pseudomonas alcaligenes |
1.13.11.4 | 7 | 9 | - |
Pseudomonas putida |
1.13.11.4 | 7 | 9 | - |
Pseudomonas alcaligenes |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.13.11.4 | Pseudomonas putida | isoelectric focusing | 4.8 | 4.6 |
1.13.11.4 | Pseudomonas alcaligenes | isoelectric focusing | - |
4.85 |