EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.9.1 | 0.001 | - |
benzyl alcohol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.005 | - |
1-butanol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.005 | - |
1-Pentanol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.005 | - |
1-Octanol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.04 | - |
octanal | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.06 | - |
1-propanol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.1 | - |
Butanal | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.2 | - |
6-aminohexan-1-ol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.28 | - |
Butane-1,3-diol | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 0.9 | - |
acetaldehyde | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 3 | - |
formaldehyde | pH 7.0, 20°C | Comamonas testosteroni | |
1.1.9.1 | 5 | - |
ethanol | pH 7.0, 20°C | Comamonas testosteroni |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.9.1 | Ca2+ | presence is essential for activity | Comamonas testosteroni |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.9.1 | 65000 | - |
1 * 65000, SDS-PAGE | Comamonas testosteroni |
1.1.9.1 | 70000 | - |
gel filtration | Comamonas testosteroni |
1.2.5.2 | 70000 | - |
gel filtration | Comamonas testosteroni |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.9.1 | Comamonas testosteroni | - |
- |
- |
1.2.5.2 | Comamonas testosteroni | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.5.2 | apoenzyme | Comamonas testosteroni |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.9.1 | -70°C, storage for prolonged periods without loss of activity | Comamonas testosteroni |
1.1.9.1 | 4°C, 0.15 M potassium phosphate buffer, pH 7.0, storage for routine purposes | Comamonas testosteroni |
1.2.5.2 | -70°C, stable for prolonged periods | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.9.1 | 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | butanal + ? | - |
r | |
1.1.9.1 | 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | octanal + ? | - |
r | |
1.1.9.1 | 1-pentanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | pentanal + ? | - |
r | |
1.1.9.1 | 1-propanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | propanal + ? | - |
r | |
1.1.9.1 | 6-aminohexan-1-ol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | 6-aminohexanal + ? | - |
r | |
1.1.9.1 | acetaldehyde + reduced N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
r | |
1.1.9.1 | benzyl alcohol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | benzaldehyde + ? | - |
r | |
1.1.9.1 | butanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
r | |
1.1.9.1 | butane-1,3-diol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | ? | - |
r | |
1.1.9.1 | ethanol + cytochrome c | 5% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 60% of the activity with Wurster's Blue | Comamonas testosteroni | ethanal + reduced cytochrome c | - |
r | |
1.1.9.1 | ethanol + ferricyanide | 100% of the activity with Wurster's Blue | Comamonas testosteroni | ethanal + ferrocyanide | - |
r | |
1.1.9.1 | ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine | i.e. Wurster's Blue | Comamonas testosteroni | ethanal + ? | - |
? | |
1.1.9.1 | ethanol + oxidized 2,6-dichlorophenolindophenol | 10% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 75% of the activity with Wurster's Blue | Comamonas testosteroni | ethanal + reduced 2,6-dichlorophenolindophenol | - |
r | |
1.1.9.1 | formaldehyde + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | methanol + ? | - |
r | |
1.1.9.1 | octanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine | - |
Comamonas testosteroni | 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine | - |
r | |
1.2.5.2 | acetaldehyde + oxidized tetramethylphenylenediamine | - |
Comamonas testosteroni | acetate + reduced tetramethylphenylenediamine | - |
? | |
1.2.5.2 | butyraldehyde + oxidized tetramethylphenylenediamine | - |
Comamonas testosteroni | butanoate + reduced tetramethylphenylenediamine | - |
? | |
1.2.5.2 | formaldehyde + oxidized tetramethylphenylenediamine | - |
Comamonas testosteroni | formate + reduced tetramethylphenylenediamine | - |
? | |
1.2.5.2 | additional information | primary alcohols (except methanol), secondary alcohols and aldehydes are substrates, and a broad range of dyes functions as artificial electron acceptor | Comamonas testosteroni | ? | - |
? | |
1.2.5.2 | octanal + oxidized tetramethylphenylenediamine | - |
Comamonas testosteroni | octanoate + reduced tetramethylphenylenediamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.9.1 | monomer | 1 * 65000, SDS-PAGE | Comamonas testosteroni |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.9.1 | quinohaemoprotein alcohol dehydrogenase | - |
Comamonas testosteroni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.9.1 | 7.7 | - |
- |
Comamonas testosteroni |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.9.1 | heme | heme group participates in enzymatic mechanism | Comamonas testosteroni | |
1.1.9.1 | pyrroloquinoline quinone | - |
Comamonas testosteroni | |
1.2.5.2 | heme | the enzyme contains one heme c group, and full reconstitution is achieved with 1 mol of pyrroloquinoline quinone/mol | Comamonas testosteroni | |
1.2.5.2 | pyrroloquinoline quinone | after reconstitution to the holoenzyme by the addition of pyrroloquinoline quinone, addition of substrate changes the absorption spectrum to that of reduced cytochrome c, indicating that the heme c group participates in the enzymic mechanism, one molecule of pyrroloquinoline quinone is bound per enzyme molecule | Comamonas testosteroni |