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Literature summary extracted from

  • Groen, B.W.; van Kleef, M.A.G.; Duine, J.A.
    Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni (1986), Biochem. J., 234, 611-615.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.9.1 0.001
-
benzyl alcohol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.005
-
1-butanol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.005
-
1-Pentanol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.005
-
1-Octanol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.04
-
octanal pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.06
-
1-propanol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.1
-
Butanal pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.2
-
6-aminohexan-1-ol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.28
-
Butane-1,3-diol pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 0.9
-
acetaldehyde pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 3
-
formaldehyde pH 7.0, 20°C Comamonas testosteroni
1.1.9.1 5
-
ethanol pH 7.0, 20°C Comamonas testosteroni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.9.1 Ca2+ presence is essential for activity Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.9.1 65000
-
1 * 65000, SDS-PAGE Comamonas testosteroni
1.1.9.1 70000
-
gel filtration Comamonas testosteroni
1.2.5.2 70000
-
gel filtration Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.1.9.1 Comamonas testosteroni
-
-
-
1.2.5.2 Comamonas testosteroni
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.2 apoenzyme Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.1.9.1 -70°C, storage for prolonged periods without loss of activity Comamonas testosteroni
1.1.9.1 4°C, 0.15 M potassium phosphate buffer, pH 7.0, storage for routine purposes Comamonas testosteroni
1.2.5.2 -70°C, stable for prolonged periods Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.9.1 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni butanal + ?
-
r
1.1.9.1 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni octanal + ?
-
r
1.1.9.1 1-pentanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni pentanal + ?
-
r
1.1.9.1 1-propanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni propanal + ?
-
r
1.1.9.1 6-aminohexan-1-ol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni 6-aminohexanal + ?
-
r
1.1.9.1 acetaldehyde + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
r
1.1.9.1 benzyl alcohol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni benzaldehyde + ?
-
r
1.1.9.1 butanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni 1-butanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
r
1.1.9.1 butane-1,3-diol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni ?
-
r
1.1.9.1 ethanol + cytochrome c 5% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 60% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced cytochrome c
-
r
1.1.9.1 ethanol + ferricyanide 100% of the activity with Wurster's Blue Comamonas testosteroni ethanal + ferrocyanide
-
r
1.1.9.1 ethanol + N,N,N',N'-tetramethyl-p-phenylenediamine i.e. Wurster's Blue Comamonas testosteroni ethanal + ?
-
?
1.1.9.1 ethanol + oxidized 2,6-dichlorophenolindophenol 10% of the activity with Wurster's Blue. In presence of phenazine methosulfate, 75% of the activity with Wurster's Blue Comamonas testosteroni ethanal + reduced 2,6-dichlorophenolindophenol
-
r
1.1.9.1 formaldehyde + N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni methanol + ?
-
r
1.1.9.1 octanal + reduced N,N,N',N'-tetramethyl-p-phenylenediamine
-
Comamonas testosteroni 1-octanol + N,N,N',N'-tetramethyl-p-phenylenediamine
-
r
1.2.5.2 acetaldehyde + oxidized tetramethylphenylenediamine
-
Comamonas testosteroni acetate + reduced tetramethylphenylenediamine
-
?
1.2.5.2 butyraldehyde + oxidized tetramethylphenylenediamine
-
Comamonas testosteroni butanoate + reduced tetramethylphenylenediamine
-
?
1.2.5.2 formaldehyde + oxidized tetramethylphenylenediamine
-
Comamonas testosteroni formate + reduced tetramethylphenylenediamine
-
?
1.2.5.2 additional information primary alcohols (except methanol), secondary alcohols and aldehydes are substrates, and a broad range of dyes functions as artificial electron acceptor Comamonas testosteroni ?
-
?
1.2.5.2 octanal + oxidized tetramethylphenylenediamine
-
Comamonas testosteroni octanoate + reduced tetramethylphenylenediamine
-
?

Subunits

EC Number Subunits Comment Organism
1.1.9.1 monomer 1 * 65000, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.1.9.1 quinohaemoprotein alcohol dehydrogenase
-
Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.9.1 7.7
-
-
Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.9.1 heme heme group participates in enzymatic mechanism Comamonas testosteroni
1.1.9.1 pyrroloquinoline quinone
-
Comamonas testosteroni
1.2.5.2 heme the enzyme contains one heme c group, and full reconstitution is achieved with 1 mol of pyrroloquinoline quinone/mol Comamonas testosteroni
1.2.5.2 pyrroloquinoline quinone after reconstitution to the holoenzyme by the addition of pyrroloquinoline quinone, addition of substrate changes the absorption spectrum to that of reduced cytochrome c, indicating that the heme c group participates in the enzymic mechanism, one molecule of pyrroloquinoline quinone is bound per enzyme molecule Comamonas testosteroni