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Literature summary extracted from

  • Shinagawa, E.; Matsushita, K.; Adachi, O.; Ameyama, M.
    Membrane-bound D-gluconate dehydrogenase of Serratia marcescens: purification and properties (1978), Agric. Biol. Chem., 42, 2355-2361.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.99.3 oxamate 1 mM, 91% loss of activity Serratia marcescens
1.1.99.3 pyruvate
-
 Serratia marcescens
1.1.99.3 Quinacrine hydrochloride 1 mM, 10% loss of activity Serratia marcescens
1.1.99.3 Quinine hydrochloride 1 mM, 42% loss of activity Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.3 0.8
-
 D-gluconate
-
 Serratia marcescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.3 membrane
-
 Serratia marcescens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.3 15500
-
 1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE Serratia marcescens
1.1.99.3 52500
-
 1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE Serratia marcescens
1.1.99.3 68000
-
 1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE Serratia marcescens
1.1.99.3 136000
-
 gel filtration Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.3 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.3
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.3 502
-
-
 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.3 D-gluconate + 2,6-dichlorophenolindophenol
-
 Serratia marcescens 2-oxogluconate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.3 trimer 1 * 15500 + 1 * 52500 + 1 * 68000, SDS-PAGE Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.3 40
-
-
 Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.99.3 35
-
 10 min, stable below Serratia marcescens
1.1.99.3 50
-
 10 min, about 50% loss of activity Serratia marcescens
1.1.99.3 60
-
 10 min, about 90% loss of activity Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.3 5
-
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.99.3 4 6 pH 4.0: about 40% of maximal activity, pH 6.0: about 60% of maximal activity Serratia marcescens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.99.3 4.5 7 5°C, 16 h, stable Serratia marcescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.3 cytochrome c1 dehydrogenase protein is tightly bound to cytochrome c1 component Serratia marcescens