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Literature summary extracted from

  • Sugimoto, M.; Tanabe, M.; Hataya, M.; Enokibara, S.; Duine, J.A.; Kawai, F.
    The first step in polyethylene glycol degradation by Sphingomonads proceeds via a flavoprotein alcohol dehydrogenase containing flavin adenine dinucleotide (2001), J. Bacteriol., 183, 6694-6698.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.99.20 gene cloned, sequenced and expressed in Escherichia coli Sphingopyxis terrae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.20 0.4
-
1-Heptanol
-
Sphingopyxis terrae
1.1.99.20 0.5
-
1-Hexanol
-
Sphingopyxis terrae
1.1.99.20 0.6
-
benzyl alcohol
-
Sphingopyxis terrae
1.1.99.20 0.7
-
PEG-400
-
Sphingopyxis terrae
1.1.99.20 0.8
-
PEG-300
-
Sphingopyxis terrae
1.1.99.20 1.6
-
PEG-1000
-
Sphingopyxis terrae
1.1.99.20 2.4
-
PEG-6000
-
Sphingopyxis terrae
1.1.99.20 4.2
-
tetraethylene glycol-monoethyl ether
-
Sphingopyxis terrae
1.1.99.20 6.6
-
1-propanol
-
Sphingopyxis terrae
1.1.99.20 14
-
Tetraethylene glycol
-
Sphingopyxis terrae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.20 membrane
-
Sphingopyxis terrae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.20 58800
-
2 * 58800, SDS-PAGE Sphingopyxis terrae
1.1.99.20 120000
-
gel filtration Sphingopyxis terrae

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.20 Sphingopyxis terrae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.20 from a symbiotic mixed culture E-1, consisting of Sphingomonas terrae and a Rhizobium sp. Sphingopyxis terrae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.20 1-heptanol + acceptor
-
Sphingopyxis terrae n-heptanal + reduced acceptor
-
?
1.1.99.20 1-hexanol + acceptor
-
Sphingopyxis terrae n-hexanal + reduced acceptor
-
?
1.1.99.20 1-propanol + 2,6-dichloro-phenolindophenol
-
Sphingopyxis terrae propanal + reduced 2,6-dichloro-phenolindophenol
-
r
1.1.99.20 aromatic alcohol + acceptor
-
Sphingopyxis terrae aromatic aldehyde + reduced acceptor
-
?
1.1.99.20 benzyl alcohol + acceptor benzyl alcohol is the best substrate Sphingopyxis terrae benzaldehyde + reduced acceptor
-
?
1.1.99.20 PEG-1000 + acceptor
-
Sphingopyxis terrae ?
-
?
1.1.99.20 PEG-300 + acceptor
-
Sphingopyxis terrae ?
-
?
1.1.99.20 PEG-400 + acceptor
-
Sphingopyxis terrae ?
-
?
1.1.99.20 PEG-6000 + acceptor
-
Sphingopyxis terrae ?
-
?
1.1.99.20 polyethylene glycol + acceptor
-
Sphingopyxis terrae ?
-
?
1.1.99.20 primary alcohol + acceptor
-
Sphingopyxis terrae aldehyde + reduced acceptor
-
?
1.1.99.20 tetraethylene glycol + acceptor
-
Sphingopyxis terrae tetraethylene glycol aldehyde + reduced acceptor
-
?
1.1.99.20 tetraethyleneglycol monoethyl ether
-
Sphingopyxis terrae ?
-
?

Subunits

EC Number Subunits Comment Organism
1.1.99.20 dimer 2 * 58800, SDS-PAGE Sphingopyxis terrae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.20 FAD
-
Sphingopyxis terrae