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Literature summary extracted from

  • Russell, R.; Scopes, R.K.
    Use of hydrophobic chromatography for purification of the membrane-located choline dehydrogenase from a Pseudomonas strain (1994), Bioseparation, 4, 279-284.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.1 2
-
 choline in the presence of 0.05 mM phenazine methosulfate Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.99.1 membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.99.1 60000 70000 gel filtration Pseudomonas aeruginosa
1.1.99.1 66000
-
 1 * 66000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.1 Pseudomonas aeruginosa
-
 C10 strain
-
1.1.99.1 Pseudomonas aeruginosa C10
-
 C10 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.1 using column chromatography on thio-hexyl and on an anion exchanger Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.99.1 67
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.1 choline + acceptor electron acceptor: phenazine methosulfate Pseudomonas aeruginosa betaine aldehyde + reduced acceptor
-
 ?
1.1.99.1 choline + acceptor electron acceptor: phenazine methosulfate Pseudomonas aeruginosa C10 betaine aldehyde + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.1 monomer 1 * 66000, SDS-PAGE Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.99.1 pyrroloquinoline quinone
-
 Pseudomonas aeruginosa