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Literature summary extracted from

  • Mendoca, M.H.; Zancan, G.T.
    Purification and characterization of intracellular galactose oxidase from Dactylium dendroides (1987), Arch. Biochem. Biophys., 252, 507-514.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.9 CN- intracellular enzyme, 37% inhibition at 0.01 mM Hypomyces rosellus
1.1.3.9 diethyldithiocarbamate intracellular enzyme, 87% inhibition at 0.005 mM Hypomyces rosellus
1.1.3.9 H2O2 42% inhibition at 2 mM Hypomyces rosellus
1.1.3.9 hydroxylamine intracellular enzyme, 47% inhibition at 0.1 mM Hypomyces rosellus
1.1.3.9 iodoacetamide 22% inhibition at 0.1 mM Hypomyces rosellus
1.1.3.9 NaN3 54% inhibition at 0.1 mM Hypomyces rosellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.9 extracellular
-
 Hypomyces rosellus
-
-
1.1.3.9 intracellular
-
 Hypomyces rosellus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.9 Cu2+
-
 Hypomyces rosellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.9 72000
-
 gel filtration, intracellular form Hypomyces rosellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.9 2-deoxy-D-galactose + O2 Hypomyces rosellus 52% of the activity compared to D-galactose 2-deoxy-D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 beta-D-lactose + O2 Hypomyces rosellus low activity beta-D-lacto-hexodialdose + H2O2
-
 ?
1.1.3.9 beta-D-lactose + O2 Hypomyces rosellus high activity beta-D-lacto-hexodialdose + H2O2
-
 ?
1.1.3.9 D-galactosamine + O2 Hypomyces rosellus
-
 ? + H2O2
-
 ?
1.1.3.9 D-galactosylpyranoside + O2 Hypomyces rosellus
-
 D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 dihydroxyacetone + O2 Hypomyces rosellus best substrate for both intra- and extracellular enzymes 3-hydroxy-2-oxo-propionaldehyde + H2O2
-
 ?
1.1.3.9 guaran + O2 Hypomyces rosellus only intracellular enzyme ? + H2O2
-
 ?
1.1.3.9 melibiose + O2 Hypomyces rosellus
-
 ? + H2O2
-
 ?
1.1.3.9 raffinose + O2 Hypomyces rosellus
-
 ? + H2O2
-
 ?
1.1.3.9 stachyose + O2 Hypomyces rosellus only intracellular enzyme ? + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.9 Hypomyces rosellus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.9 glycoprotein intracellular form highly glycosylated, extracellular form less glycosylated Hypomyces rosellus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.9 to homogeneity, intra- and extracellular forms, 1step chromatography Hypomyces rosellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.9 mycelium
-
 Hypomyces rosellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.9 116
-
 intracellular enzyme Hypomyces rosellus

Storage Stability

EC Number Storage Stability Organism
1.1.3.9 -17°C, stable for 6 months Hypomyces rosellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.9 1-O-methyl-alpha-D-galactosylpyranoside + O2
-
 Hypomyces rosellus 1-O-methyl-alpha-D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 2-deoxy-D-galactose + O2 52% of the activity compared to D-galactose Hypomyces rosellus 2-deoxy-D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 allyl alcohol + O2 only extracelluar enzyme, low activity Hypomyces rosellus acrolein + H2O2
-
 ?
1.1.3.9 beta-D-lactose + O2 low activity Hypomyces rosellus beta-D-lacto-hexodialdose + H2O2
-
 ?
1.1.3.9 beta-D-lactose + O2 high activity Hypomyces rosellus beta-D-lacto-hexodialdose + H2O2
-
 ?
1.1.3.9 beta-hydroxypyruvate + O2 only extracellular enzyme, low activity Hypomyces rosellus 2,3-dioxopropionate + H2O2
-
 ?
1.1.3.9 D-galactosamine + O2
-
 Hypomyces rosellus ? + H2O2
-
 ?
1.1.3.9 D-galactosylpyranoside + O2
-
 Hypomyces rosellus D-galacto-hexodialdose + H2O2
-
 ?
1.1.3.9 dihydroxyacetone + O2 best substrate for both intra- and extracellular enzymes Hypomyces rosellus 3-hydroxy-2-oxo-propionaldehyde + H2O2
-
 ?
1.1.3.9 glycerol + O2 only extracelluar enzyme, low activity Hypomyces rosellus (S)-glyceraldehyde + H2O2
-
 ?
1.1.3.9 glycoaldehyde + O2 only extracellular enzyme, low activity Hypomyces rosellus glyoxal + H2O2
-
 ?
1.1.3.9 guaran + O2 only intracellular enzyme Hypomyces rosellus ? + H2O2
-
 ?
1.1.3.9 melibiose + O2
-
 Hypomyces rosellus ? + H2O2
-
 ?
1.1.3.9 o-nitrophenyl beta-D-galactoside + O2
-
 Hypomyces rosellus 1-O-(o-nitrophenyl)-alpha-D-galactohexodialdose + H2O2
-
 ?
1.1.3.9 raffinose + O2
-
 Hypomyces rosellus ? + H2O2
-
 ?
1.1.3.9 stachyose + O2 only intracellular enzyme Hypomyces rosellus ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.9 monomer 1 * 72000, SDS-PAGE, intracellular form Hypomyces rosellus