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Literature summary extracted from

  • Huwel, S.; Haalck, L.; Conrath, N.; Spener, F.
    Maltose phosphorylase from Lactobacillus brevis: purification, characterization, and application in a biosensor for ortho-phosphate (1997), Enzyme Microb. Technol., 21, 413-420.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.4 analysis phosphate sensor consisting of glucose oxidase coimmobilized with glutaraldehyde with maltose phosphorylase and bovine serum albumin Aspergillus niger
2.4.1.8 biotechnology enzyme based biosensor for phosphate Levilactobacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.8 0.9
-
 maltose
-
 Levilactobacillus brevis
2.4.1.8 21.8
-
 phosphate
-
 Levilactobacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.8 196000
-
 gel filtration Levilactobacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.4 Aspergillus niger
-
-
-
2.4.1.8 Levilactobacillus brevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.8
-
 Levilactobacillus brevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.4 commercial preparation
-
 Aspergillus niger
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.8 4°C, 10 mM phosphate, pH 6.5, up to 6 months with minimal losses Levilactobacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.4 beta-D-glucose + O2 + H2O
-
 Aspergillus niger D-glucono-1,5-lactone + H2O2
-
 ?
2.4.1.8 maltose + phosphate
-
 Levilactobacillus brevis beta-D-glucose 1-phosphate + D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.8 dimer 2 x 86000, SDS-PAGE Levilactobacillus brevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.8 36
-
-
 Levilactobacillus brevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.8 6.5
-
-
 Levilactobacillus brevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.1.8 additional information no pyridoxal 5'-phosphate required Levilactobacillus brevis