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Literature summary extracted from

  • Koolman, J.
    Ecdysone oxidase (1985), Methods Enzymol., 111, 419-429.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.3.16 stable to repeated freezing and thawing Calliphora vicina

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.16 methanol 31% inhibition at 0.5% Calliphora vicina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.16 0.007
-
 oxidized 2,6-dichloroindophenol
-
 Calliphora vicina
1.1.3.16 0.031
-
 20-hydroxyecdysone
-
 Calliphora vicina
1.1.3.16 0.041
-
 ecdysone optical assay Calliphora vicina
1.1.3.16 0.098
-
 ecdysone radio assay Calliphora vicina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.3.16 cytoplasm
-
 Drosophila melanogaster 5737
-
1.1.3.16 cytoplasm
-
 Calliphora vicina 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.16 240000
-
 gel filtration Calliphora vicina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.16 20-hydroxyecdysone + O2 Calliphora vicina probably part of the catabolic system that inactivates the molting hormones 3-dehydro-20-hydroxyecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + O2 Calliphora vicina probably part of the catabolic system that inactivates the molting hormones 3-dehydroecdysone + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.16 Aeshna cyanea
-
-
-
1.1.3.16 Calliphora vicina
-
 blue blowfly
-
1.1.3.16 Choristoneura fumiferana
-
-
-
1.1.3.16 Drosophila hydei
-
-
-
1.1.3.16 Drosophila melanogaster
-
-
-
1.1.3.16 Gryllus bimaculatus
-
-
-
1.1.3.16 Locusta migratoria
-
-
-
1.1.3.16 Tenebrio molitor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.16
-
 Calliphora vicina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.16 pupa
-
 Drosophila melanogaster
-
1.1.3.16 pupa
-
 Calliphora vicina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.16 11.2
-
-
 Calliphora vicina

Storage Stability

EC Number Storage Stability Organism
1.1.3.16 -25°C, more than 3 months Calliphora vicina
1.1.3.16 0°C, several days Calliphora vicina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.16 2-deoxy-20-hydroxyecdysone + O2
-
 Calliphora vicina 3-dehydro-2-deoxy-20-hydroxyecdysone + H2O2
-
 ?
1.1.3.16 2-deoxyecdysone + O2
-
 Calliphora vicina 3-dehydro-2-deoxyecdysone + H2O2
-
 ?
1.1.3.16 20-hydroxyecdysone + O2 probably part of the catabolic system that inactivates the molting hormones Calliphora vicina 3-dehydro-20-hydroxyecdysone + H2O2
-
 ?
1.1.3.16 5,20-dihydroxyecdysone + O2
-
 Calliphora vicina 3-dehydro-5,20-dihydroxyecdysone + H2O2
-
 ?
1.1.3.16 cyasterone + O2
-
 Calliphora vicina 3-dehydrocyasterone + H2O2
-
 ?
1.1.3.16 ecdysone + O2 artificial electron acceptors in vitro in ascending order: ferricyanide, methylene blue, 2,6-dichloroindophenol, 5-methazine methyl sulfate Calliphora vicina 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 2,6-dichloroindophenol can act as acceptor Calliphora vicina 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Drosophila melanogaster 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Tenebrio molitor 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Calliphora vicina 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Locusta migratoria 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Aeshna cyanea 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Choristoneura fumiferana 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Drosophila hydei 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 i.e. 2beta,3beta,14alpha,22,25-pentahydroxy-5beta-cholest-7-en-6-one Gryllus bimaculatus 3-dehydroecdysone + H2O2 i.e. 2beta,14alpha,22,25-tetrahydroxy-5beta-cholest-7-en-3,6-dione ir
1.1.3.16 ecdysone + O2 probably part of the catabolic system that inactivates the molting hormones Calliphora vicina 3-dehydroecdysone + H2O2
-
 ?
1.1.3.16 ecdysone + oxidized 2,6-dichloroindophenol
-
 Calliphora vicina 3-dehydroecdysone + reduced 2,6-dichloroindophenol
-
 r
1.1.3.16 ecdysteroids + O2 structural requirements: alpha,beta-unsaturated keto function at C-6 and several hydroxyl functions including the hydroxyls at C-3 and at C-22 Calliphora vicina 3-dehydroecdysteroids + H2O2
-
 ?
1.1.3.16 inokosterone + O2
-
 Calliphora vicina 3-dehydroinokosterone + H2O2
-
 ?
1.1.3.16 makisterone + O2
-
 Calliphora vicina 3-dehydromakisterone + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.16 6.5
-
-
 Calliphora vicina