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Literature summary extracted from

  • Cromartie, T.H.; Walsh, C.T.
    Rat kidney L-alpha-hydroxy acid oxidase: Isolation of enzyme with one flavine coenzyme per two subunits (1975), Biochemistry, 14, 2588-2596.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 Dithionite reduction of FMN Rattus sp.
1.1.3.15 L-leucine competitive inhibition Rattus sp.
1.1.3.15 pyruvate competitive inhibition Rattus sp.
1.1.3.15 sodium sulfite reduces FMN Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 1.9
-
 L-alpha-hydroxyphenyllactate
-
 Rattus sp.
1.1.3.15 3.2
-
 DL-2-hydroxycaproate
-
 Rattus sp.
1.1.3.15 8
-
 DL-2-hydroxyisovalerate
-
 Rattus sp.
1.1.3.15 13
-
 DL-2-hydroxyvalerate
-
 Rattus sp.
1.1.3.15 14
-
 DL-2-hydroxybutyrate
-
 Rattus sp.
1.1.3.15 15
-
 L-leucine
-
 Rattus sp.
1.1.3.15 27
-
 DL-lactate
-
 Rattus sp.
1.1.3.15 28
-
 DL-3-chlorolactate
-
 Rattus sp.
1.1.3.15 40
-
 L-tryptophan
-
 Rattus sp.
1.1.3.15 53
-
 L-methionine
-
 Rattus sp.
1.1.3.15 71
-
 DL-alpha-phenyllactate
-
 Rattus sp.
1.1.3.15 90
-
 L-lysine
-
 Rattus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.15 47500
-
 4 * 47500, SDS-PAGE Rattus sp.
1.1.3.15 190000
-
 gel filtration Rattus sp.
1.1.3.15 250000
-
 sedimentation equilibrium analysis Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 DL-2-hydroxybutyrate + O2 Rattus sp.
-
 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxycaproate + O2 Rattus sp.
-
 2-oxocaproate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyisovalerate + O2 Rattus sp. low activity 2-oxoisovalerate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyvalerate + O2 Rattus sp.
-
 2-oxovalerate + H2O2
-
 ?
1.1.3.15 DL-lactate + O2 Rattus sp.
-
 pyruvate + H2O2
-
 ?
1.1.3.15 glycolate + O2 Rattus sp. preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase glyoxylate + H2O2
-
 ?
1.1.3.15 L-leucine + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-lysine + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-methionine + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-tryptophan + O2 Rattus sp.
-
 ? + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 to homogeneity, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 kidney
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 0.45
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 DL-2-hydroxybutyrate + O2
-
 Rattus sp. 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxycaproate + O2
-
 Rattus sp. 2-oxocaproate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyisovalerate + O2 low activity Rattus sp. 2-oxoisovalerate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyvalerate + O2
-
 Rattus sp. 2-oxovalerate + H2O2
-
 ?
1.1.3.15 DL-3-chlorolactate + O2 best substrate tested Rattus sp. 3-chloropyruvate + H2O2
-
 ?
1.1.3.15 DL-alpha-phenyllactate + O2
-
 Rattus sp. ? + H2O2
-
 r
1.1.3.15 DL-lactate + O2
-
 Rattus sp. pyruvate + H2O2
-
 ?
1.1.3.15 DL-phenyllactate + O2
-
 Rattus sp. phenylpyruvate + H2O2
-
 ?
1.1.3.15 glycolate + O2 preference for long chain substrates, more efficient hydroxy acid oxidase than an amino acid oxidase Rattus sp. glyoxylate + H2O2
-
 ?
1.1.3.15 L-2-hydroxyphenyllactate + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-alpha-hydroxyphenyllactate + O2
-
 Rattus sp. ? + H2O2
-
 r
1.1.3.15 L-leucine + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-lysine + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-methionine + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-tryptophan + O2
-
 Rattus sp. ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.15 tetramer 4 * 47500, SDS-PAGE Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.15 8.4
-
-
 Rattus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.15 7.5
-
 half-maximum oxidation rate Rattus sp.
1.1.3.15 9
-
 half-maximum oxidation rate Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN one FAD per two subunits Rattus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.3.15 23
-
 L-leucine
-
 Rattus sp.