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Literature summary extracted from

  • Urban, P.; Chirat, I.; Lederer, F.
    Rat kidney L-2-hydroxyacid oxidase. Structural and mechanistic comparison with flavocytochrome b2 from bakerÆs yeast (1988), Biochemistry, 27, 7365-7371.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.15 2-hydroxybutynoate inhibition of transhydrogenation reaction Rattus sp.
1.1.3.15 phosphate 50% inhibition at 0.1 M Rattus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.15 0.1
-
 DL-phenyllactate pH 7.0 and 7.5 Rattus sp.
1.1.3.15 0.15
-
 DL-2-hydroxycaproate pH 7.5 Rattus sp.
1.1.3.15 0.25
-
 DL-2-hydroxyvalerate pH 7.5 Rattus sp.
1.1.3.15 0.25
-
 DL-2-hydroxycaproate pH 7.0 Rattus sp.
1.1.3.15 0.35
-
 DL-2-hydroxyvalerate pH 7.0 Rattus sp.
1.1.3.15 0.4
-
 L-Mandelate pH 7.5 Rattus sp.
1.1.3.15 0.6
-
 DL-2-hydroxybutyrate pH 7.0 Rattus sp.
1.1.3.15 0.7
-
 L-2-Hydroxyisocaproate pH 7.5 Rattus sp.
1.1.3.15 0.7
-
 DL-3-chlorolactate pH 7.5 Rattus sp.
1.1.3.15 0.8
-
 L-Mandelate pH 7.0 Rattus sp.
1.1.3.15 0.8
-
 DL-3-chlorolactate pH 7.0 Rattus sp.
1.1.3.15 0.9
-
 L-2-Hydroxyisocaproate pH 7.0 Rattus sp.
1.1.3.15 1
-
 DL-2-hydroxybutyrate pH 7.5 Rattus sp.
1.1.3.15 1.8
-
 L-lactate pH 7.0 Rattus sp.
1.1.3.15 3.4
-
 L-lactate pH 7.5 Rattus sp.
1.1.3.15 4
-
 DL-methionine pH 7.5 Rattus sp.
1.1.3.15 4.4
-
 Bromopyruvate transhydrogenation reaction Rattus sp.
1.1.3.15 6
-
 L-leucine pH 7.5 Rattus sp.
1.1.3.15 35
-
 L-tryptophan pH 7.5 Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.15 DL-2-hydroxybutyrate + O2 Rattus sp.
-
 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxycaproate + O2 Rattus sp.
-
 2-oxocaproate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyvalerate + O2 Rattus sp.
-
 2-oxovalerate + H2O2
-
 ?
1.1.3.15 DL-methionine + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-2-hydroxyisocaproate + O2 Rattus sp.
-
 2-oxoisocaproate + H2O2
-
 ?
1.1.3.15 L-lactate + O2 Rattus sp.
-
 pyruvate + H2O2
-
 ?
1.1.3.15 L-leucine + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-mandelate + O2 Rattus sp.
-
 ? + H2O2
-
 ?
1.1.3.15 L-tryptophan + O2 Rattus sp.
-
 ? + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.15 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.15 to homogeneity, 2-step chromatography Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.15 kidney
-
 Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.15 1.2
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.15 2-hydroxybutanoate + bromopyruvate + ? transhydrogenation reaction Rattus sp. bromolactate + pyruvate + ?
-
 ?
1.1.3.15 DL-2-hydroxybutyrate + O2
-
 Rattus sp. 2-oxobutyrate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxycaproate + O2
-
 Rattus sp. 2-oxocaproate + H2O2
-
 ?
1.1.3.15 DL-2-hydroxyvalerate + O2
-
 Rattus sp. 2-oxovalerate + H2O2
-
 ?
1.1.3.15 DL-3-chlorolactate + O2
-
 Rattus sp. 3-chloropyruvate + H2O2
-
 ?
1.1.3.15 DL-methionine + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 DL-phenyllactate + O2
-
 Rattus sp. phenylpyruvate + H2O2
-
 ?
1.1.3.15 L-2-hydroxyisocaproate + O2
-
 Rattus sp. 2-oxoisocaproate + H2O2
-
 ?
1.1.3.15 L-lactate + O2
-
 Rattus sp. pyruvate + H2O2
-
 ?
1.1.3.15 L-leucine + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-mandelate + O2
-
 Rattus sp. ? + H2O2
-
 ?
1.1.3.15 L-tryptophan + O2
-
 Rattus sp. ? + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.15 0.36
-
 Bromopyruvate transhydrogenation reaction Rattus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.15 FMN one FAD per subunit Rattus sp.