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Literature summary extracted from
- D-Lactate dehydrogenase of Desulfovibrio vulgaris (1981), J. Biochem., 89, 1423-1431.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.2.4 | EDTA | slightly stimulating | Desulfovibrio vulgaris |  |
| 1.1.2.5 | EDTA | slight stimulation | Desulfovibrio vulgaris | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.2.4 | cyanide | - |
Desulfovibrio vulgaris | |
| 1.1.2.4 | EDTA | no inhibitor | Desulfovibrio vulgaris | |
| 1.1.2.4 | o-phenanthroline | very strong | Desulfovibrio vulgaris | |
| 1.1.2.4 | oxalate | - |
Desulfovibrio vulgaris | |
| 1.1.2.4 | p-chloromercuribenzoate | weak | Desulfovibrio vulgaris | |
| 1.1.2.5 | 1,10-phenanthroline | - |
Desulfovibrio vulgaris | |
| 1.1.2.5 | cyanide | - |
Desulfovibrio vulgaris | |
| 1.1.2.5 | PCMB | - |
Desulfovibrio vulgaris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.2.4 | 0.8 | - |
D-lactate | - |
Desulfovibrio vulgaris | |
| 1.1.2.5 | 0.8 | - |
D-lactate | - |
Desulfovibrio vulgaris | |
| 1.1.2.5 | 2.4 | - |
DL-2-hydroxybutyrate | - |
Desulfovibrio vulgaris | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.2.4 | additional information | equally distributed between soluble and membrane fraction | Desulfovibrio vulgaris | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.2.4 | (R)-lactate + 2 ferricytochrome c | Desulfovibrio vulgaris | required for growth on lactate as sole carbon source | pyruvate + ferrocytochrome c | - |
? | |
| 1.1.2.5 | D-lactate + ferricytochrome c-553 | Desulfovibrio vulgaris | starting enzyme for carbon and energy metabolism | pyruvate + ferrocytochrome c-553 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.2.4 | Desulfovibrio vulgaris | - |
- |
- |
| 1.1.2.5 | Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.2.4 | - |
Desulfovibrio vulgaris |
| 1.1.2.5 | partially | Desulfovibrio vulgaris |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.2.4 | 4°C, 0.1 M Tris-buffer, pH 7.3, 20% loss of activity | Desulfovibrio vulgaris |
| 1.1.2.5 | -20°C, 2 days, 20% loss of activity | Desulfovibrio vulgaris |
| 1.1.2.5 | 4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity | Desulfovibrio vulgaris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.2.4 | (R)-lactate + 2 ferricytochrome c | required for growth on lactate as sole carbon source | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c | - |
? | |
| 1.1.2.4 | (R)-lactate + ferricytochrome c | traces of activity with L-isomer | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c | - |
? | |
| 1.1.2.4 | D-2-hydroxybutyrate + cytochrome c | - |
Desulfovibrio vulgaris | 2-oxobutanoate + ferrocytochrome c | - |
? | |
| 1.1.2.5 | D-lactate + 1-methoxyphenazinium methyl sulfate | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| 1.1.2.5 | D-lactate + 2,6-dichlorophenolindophenol | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| 1.1.2.5 | D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| 1.1.2.5 | D-lactate + ferricyanide | - |
Desulfovibrio vulgaris | pyruvate + ferrocyanide | - |
? | |
| 1.1.2.5 | D-lactate + ferricytochrome c-553 | - |
Desulfovibrio vulgaris | pyruvate + ferrocytochrome c-553 | - |
? | |
| 1.1.2.5 | D-lactate + ferricytochrome c-553 | starting enzyme for carbon and energy metabolism | Desulfovibrio vulgaris | pyruvate + ferrocytochrome c-553 | - |
? | |
| 1.1.2.5 | D-lactate + methylene blue | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| 1.1.2.5 | D-lactate + nitrotetrazolium blue | - |
Desulfovibrio vulgaris | pyruvate + ? | - |
? | |
| 1.1.2.5 | DL-2-hydroxybutyrate + ferricytochrome c-553 | - |
Desulfovibrio vulgaris | 2-ketobutyrate + ferrocytochrome c-553 | - |
? | |
| 1.1.2.5 | additional information | no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity | Desulfovibrio vulgaris | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.4 | 7.5 | 9 | - |
Desulfovibrio vulgaris |
| 1.1.2.5 | 8 | - |
D-lactate + nitrotetrazolium blue | Desulfovibrio vulgaris |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.2.4 | 5 | 9 | no activity at pH 5, almost maximum activity at pH 9 | Desulfovibrio vulgaris |
| 1.1.2.5 | 7 | 9 | pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue | Desulfovibrio vulgaris |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.2.4 | 2,6-dichlorophenolindophenol | can substitute for cytochrome c | Desulfovibrio vulgaris | |
| 1.1.2.4 | cytochrome c | only cytochrome c-553 from same organism, eucaryotic cytochromes are not reduced | Desulfovibrio vulgaris | |
| 1.1.2.4 | additional information | no activity with NAD+ or NADP+ | Desulfovibrio vulgaris | |
| 1.1.2.4 | phenazine methosulfate | can substitute for cytochrome c | Desulfovibrio vulgaris | |
| 1.1.2.5 | Ferricytochrome c-553 | - |
Desulfovibrio vulgaris | |
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