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Literature summary extracted from

  • Kohn, L.D.; Packman, P.M.; Allen, R.H.; Jakoby, W.B.
    Tartaric acid metabolism. V. Crystalline tartrate dehydrogenase (1968), J. Biol. Chem., 243, 2479-2485.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.93
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.93 0.09
-
 NAD+
-
 Pseudomonas putida
1.1.1.93 0.83
-
 meso-tartrate
-
 Pseudomonas putida
1.1.1.93 1.4
-
 NADH in presence of 1.5 mM dihydroxyfumarate Pseudomonas putida
1.1.1.93 11
-
 Dihydroxyfumarate in presence of 0.19 mM NADH Pseudomonas putida
1.1.1.93 55
-
 L-Tartrate
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.93 Mn2+ required Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.93 36800
-
 4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution Pseudomonas putida
1.1.1.93 145000
-
 diffusion and sedimentation data Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.93 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.93
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.93 0.71
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.93 dihydroxyfumarate + NAD+
-
 Pseudomonas putida ?
-
 r
1.1.1.93 L-tartrate + NAD+
-
 Pseudomonas putida oxaloglycolate + NADH + H+
-
 r
1.1.1.93 meso-tartrate + NAD+
-
 Pseudomonas putida oxaloglycolate + NADH + H+
-
 r
1.1.1.93 oxaloglycolate + NADH
-
 Pseudomonas putida tartrate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.93 tetramer 4 * 36800, sedimentation study after dialysis with guanidine-mercaptoethanol solution Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.93 7
-
 dihydroxyfumarate + NADH Pseudomonas putida
1.1.1.93 8.5
-
 meso-tartrate + NAD+ Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.93 6 7.8 pH 6: about 40% of activity maximum, pH 7.8: about 80% of activity maximum, dihydroxyfumarate + NADH Pseudomonas putida
1.1.1.93 7 9 at pH 7 and pH 9: about 50% of activity maximum, mesotartrate + NAD+ Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.93 NAD+ cofactor Pseudomonas putida
1.1.1.93 NADH cofactor Pseudomonas putida