EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | NH4+ | optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides | |
1.1.1.93 | NH4+ | monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM | Cereibacter sphaeroides |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.83 | 10-40% loss of activity during repeated freezing and thawing | Cereibacter sphaeroides |
1.1.1.93 | repeated freezing and thawing of purified enzyme, 10-40% loss of activity | Cereibacter sphaeroides |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | ATP | - |
Cereibacter sphaeroides | |
1.1.1.83 | Dihydroxyfumarate | noncompetitive | Cereibacter sphaeroides | |
1.1.1.83 | iodoacetate | - |
Cereibacter sphaeroides | |
1.1.1.83 | L-Tartrate | competitive with respect to D-malate | Cereibacter sphaeroides | |
1.1.1.83 | meso-tartrate | competitive | Cereibacter sphaeroides | |
1.1.1.83 | oxaloacetate | competitive | Cereibacter sphaeroides | |
1.1.1.93 | ATP | - |
Cereibacter sphaeroides | |
1.1.1.93 | Dihydroxyfumarate | - |
Cereibacter sphaeroides | |
1.1.1.93 | meso-tartrate | competitive | Cereibacter sphaeroides | |
1.1.1.93 | oxaloacetate | competitive | Cereibacter sphaeroides |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.83 | 0.13 | - |
NAD+ | - |
Cereibacter sphaeroides | |
1.1.1.83 | 0.17 | - |
D-malate | - |
Cereibacter sphaeroides | |
1.1.1.93 | 0.13 | - |
NAD+ | reaction with D-malate | Cereibacter sphaeroides | |
1.1.1.93 | 0.17 | - |
D-malate | - |
Cereibacter sphaeroides | |
1.1.1.93 | 0.28 | - |
NAD+ | reaction with (+ L-(+)-tartrate) | Cereibacter sphaeroides | |
1.1.1.93 | 2.3 | - |
L-Tartrate | - |
Cereibacter sphaeroides |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | Co2+ | 1 mM, 30% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.83 | K2SO4 | 50 mM, 56% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.83 | KCl | 50 mM, 63% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.83 | Mg2+ | 5 mM, 59% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.83 | Mn2+ | Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+ | Cereibacter sphaeroides | |
1.1.1.83 | NaCl | 50 mM, 19% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.83 | Zn2+ | 0.1 mM, 14% of the activity with Mn2+ | Cereibacter sphaeroides | |
1.1.1.93 | Co2+ | 1 mM, 30% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides | |
1.1.1.93 | K+ | 50 mM KCl, 63% of the activation with 50 mM NH4Cl | Cereibacter sphaeroides | |
1.1.1.93 | K+ | 50 mM K2SO4, 56% of the activation with 50 mM KCl | Cereibacter sphaeroides | |
1.1.1.93 | Mg2+ | 5 mM, 59% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides | |
1.1.1.93 | Mn2+ | Km: 0.016 mM, reaction with L-malate or D-malate | Cereibacter sphaeroides | |
1.1.1.93 | Mn2+ | monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM | Cereibacter sphaeroides | |
1.1.1.93 | NaCl | 50 mM KCl, 19% of the activation with 50 mM NH4Cl | Cereibacter sphaeroides | |
1.1.1.93 | Zn2+ | 0.1 mM, 14% of the activation with 0.4 mM MnCl2 | Cereibacter sphaeroides |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 38500 | - |
4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
1.1.1.83 | 158000 | - |
gel filtration | Cereibacter sphaeroides |
1.1.1.83 | 162000 | - |
equilibrium sedimentation | Cereibacter sphaeroides |
1.1.1.93 | 38500 | - |
4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
1.1.1.93 | 158000 | - |
gel filtration | Cereibacter sphaeroides |
1.1.1.93 | 162000 | - |
ultracentrifugation | Cereibacter sphaeroides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.83 | Cereibacter sphaeroides | - |
- |
- |
1.1.1.83 | Cereibacter sphaeroides Y | - |
- |
- |
1.1.1.93 | Cereibacter sphaeroides | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.83 | bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase | Cereibacter sphaeroides |
1.1.1.93 | bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating), EC 1.1.1.93/EC 1.1.1.83 | Cereibacter sphaeroides |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 20.6 | - |
- |
Cereibacter sphaeroides |
1.1.1.93 | 1.88 | - |
- |
Cereibacter sphaeroides |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.83 | -20°C, stable for months | Cereibacter sphaeroides |
1.1.1.83 | 0°C, stable for 30 h, even at protein concentration below 1 mg/ml | Cereibacter sphaeroides |
1.1.1.93 | -20°C, months, partially purified enzyme | Cereibacter sphaeroides |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.83 | (R)-malate + NAD+ | - |
Cereibacter sphaeroides | pyruvate + CO2 + NADH | - |
? | |
1.1.1.83 | (R)-malate + NAD+ | - |
Cereibacter sphaeroides Y | pyruvate + CO2 + NADH | - |
? | |
1.1.1.83 | additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides | ? | - |
? | |
1.1.1.83 | additional information | bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate | Cereibacter sphaeroides Y | ? | - |
? | |
1.1.1.93 | D-malate + NAD+ | - |
Cereibacter sphaeroides | pyruvate + CO2 + NADH + H+ | - |
r | |
1.1.1.93 | D-malate + NAD+ | - |
Cereibacter sphaeroides Y | pyruvate + CO2 + NADH + H+ | - |
r | |
1.1.1.93 | L-tartrate + NAD+ | - |
Cereibacter sphaeroides | oxaloglycolate + NADH + H+ | dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate | ? | |
1.1.1.93 | L-tartrate + NAD+ | - |
Cereibacter sphaeroides Y | oxaloglycolate + NADH + H+ | dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.83 | tetramer | 4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
1.1.1.93 | tetramer | 4 * 38500, SDS-PAGE | Cereibacter sphaeroides |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 50 | - |
- |
Cereibacter sphaeroides |
1.1.1.93 | 50 | - |
- |
Cereibacter sphaeroides |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 30 | - |
half-life: 30 h | Cereibacter sphaeroides |
1.1.1.83 | 50 | - |
half-life: 120 min | Cereibacter sphaeroides |
1.1.1.83 | 55 | - |
half-life: 30 min | Cereibacter sphaeroides |
1.1.1.83 | 60 | - |
half-life: 9 min | Cereibacter sphaeroides |
1.1.1.93 | 30 | - |
pH 7.0, 30 h: 50% loss of activity. pH 8.5, stable for 4 h | Cereibacter sphaeroides |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 8.4 | 9 | - |
Cereibacter sphaeroides |
1.1.1.93 | 8.4 | 9 | bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 | Cereibacter sphaeroides |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 7 | 10.6 | at pH 7.0 and 10.6: about 50% of activity maximum | Cereibacter sphaeroides |
1.1.1.93 | 7 | 10.6 | at pH 7.0 and pH 10.6: 50% of activity maximum | Cereibacter sphaeroides |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.83 | 8.5 | - |
30°C, 4 h, stable | Cereibacter sphaeroides |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.83 | NAD+ | - |
Cereibacter sphaeroides | |
1.1.1.93 | NAD+ | cofactor | Cereibacter sphaeroides |