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Literature summary extracted from

  • Giffhorn, F.; Kuhn, A.
    Purification and characterization of a bifunctional L-(+)-tartrate dehydrogenase-D-(+)-malate dehydrogenase (decarboxylating) from Rhodopseudomonas sphaeroides Y (1983), J. Bacteriol., 155, 281-290.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.83 NH4+ optimal activity in presence of both Mn2+ and NH4+ Cereibacter sphaeroides
1.1.1.93 NH4+ monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM Cereibacter sphaeroides

General Stability

EC Number General Stability Organism
1.1.1.83 10-40% loss of activity during repeated freezing and thawing Cereibacter sphaeroides
1.1.1.93 repeated freezing and thawing of purified enzyme, 10-40% loss of activity Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.83 ATP
-
Cereibacter sphaeroides
1.1.1.83 Dihydroxyfumarate noncompetitive Cereibacter sphaeroides
1.1.1.83 iodoacetate
-
Cereibacter sphaeroides
1.1.1.83 L-Tartrate competitive with respect to D-malate Cereibacter sphaeroides
1.1.1.83 meso-tartrate competitive Cereibacter sphaeroides
1.1.1.83 oxaloacetate competitive Cereibacter sphaeroides
1.1.1.93 ATP
-
Cereibacter sphaeroides
1.1.1.93 Dihydroxyfumarate
-
Cereibacter sphaeroides
1.1.1.93 meso-tartrate competitive Cereibacter sphaeroides
1.1.1.93 oxaloacetate competitive Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.83 0.13
-
NAD+
-
Cereibacter sphaeroides
1.1.1.83 0.17
-
D-malate
-
Cereibacter sphaeroides
1.1.1.93 0.13
-
NAD+ reaction with D-malate Cereibacter sphaeroides
1.1.1.93 0.17
-
D-malate
-
Cereibacter sphaeroides
1.1.1.93 0.28
-
NAD+ reaction with (+ L-(+)-tartrate) Cereibacter sphaeroides
1.1.1.93 2.3
-
L-Tartrate
-
Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.83 Co2+ 1 mM, 30% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.83 K2SO4 50 mM, 56% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.83 KCl 50 mM, 63% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.83 Mg2+ 5 mM, 59% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.83 Mn2+ Km: 0.016 mM, optimal activity in presence of both Mn2+ and NH4+ Cereibacter sphaeroides
1.1.1.83 NaCl 50 mM, 19% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.83 Zn2+ 0.1 mM, 14% of the activity with Mn2+ Cereibacter sphaeroides
1.1.1.93 Co2+ 1 mM, 30% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides
1.1.1.93 K+ 50 mM KCl, 63% of the activation with 50 mM NH4Cl Cereibacter sphaeroides
1.1.1.93 K+ 50 mM K2SO4, 56% of the activation with 50 mM KCl Cereibacter sphaeroides
1.1.1.93 Mg2+ 5 mM, 59% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides
1.1.1.93 Mn2+ Km: 0.016 mM, reaction with L-malate or D-malate Cereibacter sphaeroides
1.1.1.93 Mn2+ monovalent and divalent cations are essential for optimal activity. At saturating concentrations of 0.4 mM MnCl2 ammonium sulfate stimulates optimally over a broad concentration range, from 40 mM to 100 mM Cereibacter sphaeroides
1.1.1.93 NaCl 50 mM KCl, 19% of the activation with 50 mM NH4Cl Cereibacter sphaeroides
1.1.1.93 Zn2+ 0.1 mM, 14% of the activation with 0.4 mM MnCl2 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.83 38500
-
4 * 38500, SDS-PAGE Cereibacter sphaeroides
1.1.1.83 158000
-
gel filtration Cereibacter sphaeroides
1.1.1.83 162000
-
equilibrium sedimentation Cereibacter sphaeroides
1.1.1.93 38500
-
4 * 38500, SDS-PAGE Cereibacter sphaeroides
1.1.1.93 158000
-
gel filtration Cereibacter sphaeroides
1.1.1.93 162000
-
ultracentrifugation Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.83 Cereibacter sphaeroides
-
-
-
1.1.1.83 Cereibacter sphaeroides Y
-
-
-
1.1.1.93 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.83 bifunctional L-(+)-tartrate-D-(+)-malate dehydrogenase Cereibacter sphaeroides
1.1.1.93 bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating), EC 1.1.1.93/EC 1.1.1.83 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.83 20.6
-
-
Cereibacter sphaeroides
1.1.1.93 1.88
-
-
Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
1.1.1.83 -20°C, stable for months Cereibacter sphaeroides
1.1.1.83 0°C, stable for 30 h, even at protein concentration below 1 mg/ml Cereibacter sphaeroides
1.1.1.93 -20°C, months, partially purified enzyme Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.83 (R)-malate + NAD+
-
Cereibacter sphaeroides pyruvate + CO2 + NADH
-
?
1.1.1.83 (R)-malate + NAD+
-
Cereibacter sphaeroides Y pyruvate + CO2 + NADH
-
?
1.1.1.83 additional information bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate Cereibacter sphaeroides ?
-
?
1.1.1.83 additional information bifunctional L(+)-tartrate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 also catalyzes nonoxidative decarboxylation of L-tartrate Cereibacter sphaeroides Y ?
-
?
1.1.1.93 D-malate + NAD+
-
Cereibacter sphaeroides pyruvate + CO2 + NADH + H+
-
r
1.1.1.93 D-malate + NAD+
-
Cereibacter sphaeroides Y pyruvate + CO2 + NADH + H+
-
r
1.1.1.93 L-tartrate + NAD+
-
Cereibacter sphaeroides oxaloglycolate + NADH + H+ dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate ?
1.1.1.93 L-tartrate + NAD+
-
Cereibacter sphaeroides Y oxaloglycolate + NADH + H+ dihydroxyfumarate is in tautomeric equilibrium with oxaloglycolate ?

Subunits

EC Number Subunits Comment Organism
1.1.1.83 tetramer 4 * 38500, SDS-PAGE Cereibacter sphaeroides
1.1.1.93 tetramer 4 * 38500, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.83 50
-
-
Cereibacter sphaeroides
1.1.1.93 50
-
-
Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.83 30
-
half-life: 30 h Cereibacter sphaeroides
1.1.1.83 50
-
half-life: 120 min Cereibacter sphaeroides
1.1.1.83 55
-
half-life: 30 min Cereibacter sphaeroides
1.1.1.83 60
-
half-life: 9 min Cereibacter sphaeroides
1.1.1.93 30
-
pH 7.0, 30 h: 50% loss of activity. pH 8.5, stable for 4 h Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.83 8.4 9
-
Cereibacter sphaeroides
1.1.1.93 8.4 9 bifunctional L-(+)-tartrate dehydrogenase/D-(+)-malate dehydrogenase (decarboxylating) EC 1.1.1.93/EC 1.1.1.83 Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.83 7 10.6 at pH 7.0 and 10.6: about 50% of activity maximum Cereibacter sphaeroides
1.1.1.93 7 10.6 at pH 7.0 and pH 10.6: 50% of activity maximum Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.83 8.5
-
30°C, 4 h, stable Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.83 NAD+
-
Cereibacter sphaeroides
1.1.1.93 NAD+ cofactor Cereibacter sphaeroides