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Literature summary extracted from
- Purification and characterisation of TOL plasmid-encoded benzyl alcohol dehydrogenase and benzaldehyde dehydrogenase of Pseudomonas putida (1990), Eur. J. Biochem., 191, 705-714.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.90 | 2-mercaptoethanol stabilizes | Pseudomonas putida |
| 1.1.1.90 | benzyl alcohol stabilizes slightly | Pseudomonas putida |
| 1.1.1.90 | dithiothreitol stabilizes | Pseudomonas putida |
| 1.1.1.90 | MgCl2 stabilizes slightly | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.90 | dithiothreitol | above 2.5 mM | Pseudomonas putida |  |
| 1.1.1.90 | iodoacetamide | slight | Pseudomonas putida | |
| 1.1.1.90 | N-ethylmaleimide | - |
Pseudomonas putida | |
| 1.1.1.90 | NADH | noncompetitive | Pseudomonas putida | |
| 1.1.1.90 | p-chloromercuribenzoate | - |
Pseudomonas putida | |
| 1.1.1.90 | SH-blocking agents | - |
Pseudomonas putida | |
| 1.2.1.32 | iodoacetamide | equimolar amount reduces benzaldehyde oxidation activity by 44% | Pseudomonas putida | |
| 1.2.1.32 | N-ethylmaleimide | equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity | Pseudomonas putida | |
| 1.2.1.32 | p-chloromercuribenzoate | equimolar amount inactivates benzaldehyde oxidation completely, 5 mM 2-mercaptoethanol restores activity | Pseudomonas putida | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.90 | 0.0257 | - |
benzaldehyde | - |
Pseudomonas putida | |
| 1.1.1.90 | 0.047 | - |
NADH | - |
Pseudomonas putida | |
| 1.1.1.90 | 0.0476 | - |
NAD+ | - |
Pseudomonas putida | |
| 1.1.1.90 | 0.2194 | - |
benzyl alcohol | - |
Pseudomonas putida | |
| 1.2.1.32 | 0.46 | - |
benzaldehyde | - |
Pseudomonas putida | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.90 | KBr | - |
Pseudomonas putida | |
| 1.1.1.90 | KCl | activity nearly doubled | Pseudomonas putida | |
| 1.1.1.90 | KNO3 | - |
Pseudomonas putida | |
| 1.1.1.90 | MgCl2 | slight activation | Pseudomonas putida | |
| 1.1.1.90 | NaCl | - |
Pseudomonas putida | |
| 1.1.1.90 | NH4Cl | - |
Pseudomonas putida | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 42000 | - |
2 * 42000, SDS-PAGE | Pseudomonas putida |
| 1.1.1.90 | 90000 | - |
gel filtration | Pseudomonas putida |
| 1.2.1.32 | 57000 | - |
2 * 57000, SDS-PAGE | Pseudomonas putida |
| 1.2.1.32 | 126000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.90 | benzyl alcohol + NAD+ | Pseudomonas putida | - |
benzaldehyde + NADH | - |
r | |
| 1.1.1.90 | benzyl alcohol + NAD+ | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
benzaldehyde + NADH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.90 | Pseudomonas putida | - |
- |
- |
| 1.2.1.32 | Pseudomonas putida | - |
carrying the TOL plasmid pWW0 | - |
| 1.2.1.32 | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | - |
carrying the TOL plasmid pWW0 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.90 | enzyme encoded by plasmid TOL | Pseudomonas putida |
| 1.2.1.32 | erroneously identified as benzaldehyde dehydrogenase | Pseudomonas putida |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 230.3 | - |
- |
Pseudomonas putida |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.90 | -70°C, enzyme dialyzed against buffer containing 40% glycerol | Pseudomonas putida |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.90 | 2-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 2-aminobenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 2-hydroxymethylthiophene + NAD+ | - |
Pseudomonas putida | 2-formylthiophene + NADH | - |
r | |
| 1.1.1.90 | 2-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 2-methylbenzaldehyde + NADH + H+ | - |
r | |
| 1.1.1.90 | 2-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | 2-methylbenzaldehyde + NADH + H+ | - |
r | |
| 1.1.1.90 | 3,4-dimethylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3,4-dimethylbenzaldehyde + NADH + H+ | - |
r | |
| 1.1.1.90 | 3-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-aminobenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 3-chlorobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-chlorobenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 3-hydroxybenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-hydroxybenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 3-methoxybenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-methoxybenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 3-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-methylbenzaldehyde + NADH + H+ | - |
r | |
| 1.1.1.90 | 3-nitrobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 3-nitrobenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 4-aminobenzyl alcohol + NAD+ | - |
Pseudomonas putida | 4-aminobenzaldehyde + NADH | - |
r | |
| 1.1.1.90 | 4-methylbenzyl alcohol + NAD+ | - |
Pseudomonas putida | 4-methylbenzaldehyde + NADH + H+ | - |
r | |
| 1.1.1.90 | benzyl alcohol + NAD+ | - |
Pseudomonas putida | benzaldehyde + NADH | - |
r | |
| 1.1.1.90 | benzyl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | benzaldehyde + NADH | - |
r | |
| 1.1.1.90 | furfuryl alcohol + NAD+ | - |
Pseudomonas putida | furfural + NADH | - |
r | |
| 1.1.1.90 | furfuryl alcohol + NAD+ | - |
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | furfural + NADH | - |
r | |
| 1.1.1.90 | additional information | not: 4-nitrophenyl acetate | Pseudomonas putida | ? | - |
? | |
| 1.1.1.90 | additional information | a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols | Pseudomonas putida | ? | - |
? | |
| 1.1.1.90 | additional information | not: 4-nitrophenyl acetate | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | ? | - |
? | |
| 1.1.1.90 | additional information | a group of enzymes with broad specificity towards alcohols with an aromatic or cyclohex-1-ene ring, but with low or no activity towards short chain aliphatic alcohols | Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 | ? | - |
? | |
| 1.2.1.32 | 3-chlorobenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-chlorobenzoate + NADH | - |
? | |
| 1.2.1.32 | 3-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 3-methylbenzoate + NADH | - |
? | |
| 1.2.1.32 | 3-nitrobenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 3-nitrobenzoate + NADH | - |
? | |
| 1.2.1.32 | 4-carboxybenzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | 4-carboxybenzoate + NADH | - |
? | |
| 1.2.1.32 | 4-methoxybenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 4-methoxybenzoate + NADH + H+ | - |
? | |
| 1.2.1.32 | 4-methylbenzaldehyde + NAD+ + H2O | low activity | Pseudomonas putida | 4-methylbenzoate + NADH | - |
? | |
| 1.2.1.32 | benzaldehyde + NAD+ + H2O | - |
Pseudomonas putida | benzoate + NADH + H+ | - |
? | |
| 1.2.1.32 | additional information | no reaction with 2-methylbenzaldehyde | Pseudomonas putida | ? | - |
? | |
| 1.2.1.32 | additional information | no reaction with 2-hydroxybenzaldehyde, 3-hydroxybenzaldehyde | Pseudomonas putida | ? | - |
? | |
| 1.2.1.32 | pyridine 4-carboxyaldehyde + NAD+ + H2O | - |
Pseudomonas putida | pyridine 4-carboxylate + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.90 | dimer | 2 * 42000, SDS-PAGE | Pseudomonas putida |
| 1.2.1.32 | dimer | 2 * 57000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 25 | - |
assay at | Pseudomonas putida |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 37 | - |
after 21 h no loss of activity | Pseudomonas putida |
| 1.1.1.90 | 56 | - |
after 10 min complete loss of activity | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.90 | 228 | - |
benzyl alcohol | - |
Pseudomonas putida | |
| 1.1.1.90 | 622 | - |
benzaldehyde | - |
Pseudomonas putida | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 9.4 | - |
- |
Pseudomonas putida |
| 1.2.1.32 | 9.6 | - |
benzaldehyde oxidation | Pseudomonas putida |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.90 | 7.5 | 10.5 | pH 7.5: about 15% of activity maximum, pH 10.5: about 20% of activity maximum | Pseudomonas putida |
| 1.2.1.32 | 8 | 11 | benzaldehyde oxidation, more than 50% of maximal activity between pH 8 and 11 | Pseudomonas putida |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.90 | additional information | contains no prosthetic group such as FAD or FMN | Pseudomonas putida | |
| 1.1.1.90 | NAD+ | enzyme transfers hydride to the pro-R side of the prochiral C4 to the pyridine ring of NAD+ | Pseudomonas putida | |
| 1.1.1.90 | NADH | - |
Pseudomonas putida | |
| 1.2.1.32 | NAD+ | - |
Pseudomonas putida | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.90 | 0.8 | - |
NADH | - |
Pseudomonas putida | |
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