BRENDA - Enzyme Database

Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea

Seitz, H.U.; Gaertner, D.E.; Plant Cell Tissue Organ Cult. 38, 337-344 (1994)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.50
0.235
-
5beta-Pregnan-3alpha-ol-20-one
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
0.4
-
NADH
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
0.91
-
NADPH
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
1.33
-
NADPH
microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.277
0.00139
-
NADH
-
Digitalis purpurea
1.1.1.277
0.0171
-
5beta-pregnane-3,20-dione
-
Digitalis purpurea
1.1.1.277
0.3
-
NADPH
-
Digitalis purpurea
1.3.1.22
0.03
-
progesterone
-
Digitalis purpurea
1.3.1.22
0.13
-
NADPH
-
Digitalis purpurea
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.50
cytosol
NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, 97% in the cytosol
Digitalis purpurea
5829
-
1.1.1.50
microsome
low activity of NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
Digitalis purpurea
-
-
1.1.1.277
soluble
-
Digitalis purpurea
-
-
1.3.1.22
endoplasmic reticulum
-
Digitalis purpurea
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.50
5alpha-pregnan-3,20-dione + NAD(P)H + H+
Digitalis purpurea
5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors
5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
Digitalis purpurea
?
1.1.1.50
5beta-pregnan-3,20-dione + NAD(P)H
Digitalis purpurea
3alpha-hydroxysteroid-5beta-oxidoreductase activity
3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
Digitalis purpurea
biosynthesis of cardenolides
5beta-pregnane-3,20-dione + NADPH
-
-
-
1.3.1.22
progesterone + NADPH
Digitalis purpurea
-
5alpha-pregnan-3-20-dione + NADP+
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.1.1.50
Digitalis purpurea
-
-
-
1.1.1.277
Digitalis purpurea
-
-
-
1.3.1.22
Digitalis purpurea
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.50
shoot
culture
Digitalis purpurea
-
1.1.1.277
additional information
no activity in undifferentiated cell cultures
Digitalis purpurea
-
1.1.1.277
shoot
-
Digitalis purpurea
-
1.3.1.22
shoot
-
Digitalis purpurea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.1.1.50
3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
389560
Digitalis purpurea
5beta-pregnan-3,20-dione + NADPH
-
-
-
r
1.1.1.50
5alpha-pregnan-3,20-dione + NAD(P)H + H+
5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors
389560
Digitalis purpurea
5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
389560
Digitalis purpurea
?
1.1.1.50
5beta-pregnan-3,20-dione + NAD(P)H
3alpha-hydroxysteroid-5beta-oxidoreductase activity
389560
Digitalis purpurea
3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
389560
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
stereospecific
389560
Digitalis purpurea
5beta-pregnane-3,20-dione + NADPH
-
389560
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
biosynthesis of cardenolides
389560
Digitalis purpurea
5beta-pregnane-3,20-dione + NADPH
-
-
-
-
1.3.1.22
progesterone + NADPH
-
389560
Digitalis purpurea
5alpha-pregnan-3-20-dione + NADP+
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.1.1.50
3alpha-hydroxysteroid-5beta-oxidoreductase activity
-
Digitalis purpurea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.50
NAD+
-
Digitalis purpurea
1.1.1.50
NADH
cytosolic 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
Digitalis purpurea
1.1.1.50
NADPH
NADPH-linked microsomal 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase shows very low activity only with NADPH
Digitalis purpurea
1.1.1.277
NADH
affinity to NADH is lower than to NADPH
Digitalis purpurea
1.1.1.277
NADPH
-
Digitalis purpurea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.50
NAD+
-
Digitalis purpurea
1.1.1.50
NADH
cytosolic 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
Digitalis purpurea
1.1.1.50
NADPH
NADPH-linked microsomal 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase shows very low activity only with NADPH
Digitalis purpurea
1.1.1.277
NADH
affinity to NADH is lower than to NADPH
Digitalis purpurea
1.1.1.277
NADPH
-
Digitalis purpurea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.50
0.235
-
5beta-Pregnan-3alpha-ol-20-one
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
0.4
-
NADH
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
0.91
-
NADPH
cytosolic NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.50
1.33
-
NADPH
microsomal NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase
Digitalis purpurea
1.1.1.277
0.00139
-
NADH
-
Digitalis purpurea
1.1.1.277
0.0171
-
5beta-pregnane-3,20-dione
-
Digitalis purpurea
1.1.1.277
0.3
-
NADPH
-
Digitalis purpurea
1.3.1.22
0.03
-
progesterone
-
Digitalis purpurea
1.3.1.22
0.13
-
NADPH
-
Digitalis purpurea
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.50
cytosol
NADH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, 97% in the cytosol
Digitalis purpurea
5829
-
1.1.1.50
microsome
low activity of NADPH-linked 5beta-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity
Digitalis purpurea
-
-
1.1.1.277
soluble
-
Digitalis purpurea
-
-
1.3.1.22
endoplasmic reticulum
-
Digitalis purpurea
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.1.1.50
5alpha-pregnan-3,20-dione + NAD(P)H + H+
Digitalis purpurea
5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors
5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
Digitalis purpurea
?
1.1.1.50
5beta-pregnan-3,20-dione + NAD(P)H
Digitalis purpurea
3alpha-hydroxysteroid-5beta-oxidoreductase activity
3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
Digitalis purpurea
biosynthesis of cardenolides
5beta-pregnane-3,20-dione + NADPH
-
-
-
1.3.1.22
progesterone + NADPH
Digitalis purpurea
-
5alpha-pregnan-3-20-dione + NADP+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.50
shoot
culture
Digitalis purpurea
-
1.1.1.277
additional information
no activity in undifferentiated cell cultures
Digitalis purpurea
-
1.1.1.277
shoot
-
Digitalis purpurea
-
1.3.1.22
shoot
-
Digitalis purpurea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.1.1.50
3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
389560
Digitalis purpurea
5beta-pregnan-3,20-dione + NADPH
-
-
-
r
1.1.1.50
5alpha-pregnan-3,20-dione + NAD(P)H + H+
5alpha-dihydroprogesterone 3alpha-hydroxysteroid oxidoreductase activity, NADPH and NADH possible as cofactors
389560
Digitalis purpurea
5alpha-pregnane-3alpha-ol-20-one + NAD(P)+
-
389560
Digitalis purpurea
?
1.1.1.50
5beta-pregnan-3,20-dione + NAD(P)H
3alpha-hydroxysteroid-5beta-oxidoreductase activity
389560
Digitalis purpurea
3alpha-hydroxy-5beta-pregnan-20-one + NAD(P)+
-
389560
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
stereospecific
389560
Digitalis purpurea
5beta-pregnane-3,20-dione + NADPH
-
389560
Digitalis purpurea
r
1.1.1.277
3beta-hydroxy-5beta-pregnane-20-one + NADP+
biosynthesis of cardenolides
389560
Digitalis purpurea
5beta-pregnane-3,20-dione + NADPH
-
-
-
-
1.3.1.22
progesterone + NADPH
-
389560
Digitalis purpurea
5alpha-pregnan-3-20-dione + NADP+
-
-
-
?