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Literature summary extracted from

  • Gao, X.; Liu, J.; Li, G.
    Purification and properties of 3alpha-hydroxysteroid dehydrogenase and 3-keto-5beta-steroid-DELTA4-dehydrogenase (1998), Ann. N. Y. Acad. Sci., 864, 383-392.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.50 medicine enzyme as part of commercial enzyme test kit for bile acid content in serum as an index for hepatic diseases Pseudomonas sp.
1.3.99.6 analysis determination of the concentration of chenodeoxycholic acid by an enzyme kit consisting of 3alpha-hydroxysteroid dehydrogenase, 3-keto-5beta-steroid-DELTA4-dehydrogenase, NAD+, nitroblue tetrazolium, diaphorase and some other reagents Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.64
-
NAD+
-
Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.50 cytosol
-
Pseudomonas sp. 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 47000
-
gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 3-hydroxysteroid + NAD(P)+ Pseudomonas sp.
-
3-ketosteroid + NAD(P)H
-
?
1.3.99.6 additional information Pseudomonas sp. inducible by testosterone and progesterone ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas sp.
-
-
-
1.3.99.6 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.50
-
Pseudomonas sp.
1.3.99.6
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.6 additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3-hydroxysteroid + NAD(P)+
-
Pseudomonas sp. 3-ketosteroid + NAD(P)H
-
?
1.3.99.6 5beta-androstan-3,17-dione + acceptor acceptor: nitroblue tetrazolium Pseudomonas sp. 4-androsten-3,17-dione + reduced acceptor
-
?
1.3.99.6 additional information inducible by testosterone and progesterone Pseudomonas sp. ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 37
-
-
Pseudomonas sp.
1.3.99.6 37
-
-
Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.50 28 46
-
Pseudomonas sp.
1.3.99.6 31 43 31°C: about 70% of maximal activity, 43°C: about 60% of maximal activity Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 10
-
oxidation of 3alpha-hydroxysteroids Pseudomonas sp.
1.3.99.6 8.5 10.5 pH 8.5: about 40% of maximal activity, pH 10.5: about 20% of maximal activity Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.50 8.5 11
-
Pseudomonas sp.
1.3.99.6 9.5
-
-
Pseudomonas sp.