EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.50 | medicine | enzyme as part of commercial enzyme test kit for bile acid content in serum as an index for hepatic diseases | Pseudomonas sp. |
1.3.99.6 | analysis | determination of the concentration of chenodeoxycholic acid by an enzyme kit consisting of 3alpha-hydroxysteroid dehydrogenase, 3-keto-5beta-steroid-DELTA4-dehydrogenase, NAD+, nitroblue tetrazolium, diaphorase and some other reagents | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.50 | 0.64 | - |
NAD+ | - |
Pseudomonas sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.50 | cytosol | - |
Pseudomonas sp. | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 47000 | - |
gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 3-hydroxysteroid + NAD(P)+ | Pseudomonas sp. | - |
3-ketosteroid + NAD(P)H | - |
? | |
1.3.99.6 | additional information | Pseudomonas sp. | inducible by testosterone and progesterone | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.50 | Pseudomonas sp. | - |
- |
- |
1.3.99.6 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.50 | - |
Pseudomonas sp. |
1.3.99.6 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.99.6 | additional information | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.50 | 3-hydroxysteroid + NAD(P)+ | - |
Pseudomonas sp. | 3-ketosteroid + NAD(P)H | - |
? | |
1.3.99.6 | 5beta-androstan-3,17-dione + acceptor | acceptor: nitroblue tetrazolium | Pseudomonas sp. | 4-androsten-3,17-dione + reduced acceptor | - |
? | |
1.3.99.6 | additional information | inducible by testosterone and progesterone | Pseudomonas sp. | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 37 | - |
- |
Pseudomonas sp. |
1.3.99.6 | 37 | - |
- |
Pseudomonas sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 28 | 46 | - |
Pseudomonas sp. |
1.3.99.6 | 31 | 43 | 31°C: about 70% of maximal activity, 43°C: about 60% of maximal activity | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 10 | - |
oxidation of 3alpha-hydroxysteroids | Pseudomonas sp. |
1.3.99.6 | 8.5 | 10.5 | pH 8.5: about 40% of maximal activity, pH 10.5: about 20% of maximal activity | Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.50 | 8.5 | 11 | - |
Pseudomonas sp. |
1.3.99.6 | 9.5 | - |
- |
Pseudomonas sp. |