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Literature summary extracted from

  • Obata, H.; Uebayasi, M.; Kaneda, T.
    Purification and properties of 4-hydroxycyclohexanecarboxylate dehydrogenase from Corynebacterium cyclohexanicum (1988), Eur. J. Biochem., 174, 451-458.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.226 iodoacetamide slight inhibition Corynebacterium cyclohexanicum
1.1.1.226 iodoacetic acid slight inhibition Corynebacterium cyclohexanicum
1.1.1.226 N-bromosuccinimide strong inhibition Corynebacterium cyclohexanicum
1.1.1.226 p-hydroxymercuribenzoate slight inhibition Corynebacterium cyclohexanicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.226 0.23
-
 NAD+
-
 Corynebacterium cyclohexanicum
1.1.1.226 0.28
-
 NADH
-
 Corynebacterium cyclohexanicum
1.1.1.226 0.5
-
 4-Oxocyclohexanecarboxylate
-
 Corynebacterium cyclohexanicum
1.1.1.226 0.51
-
 trans-4-hydroxycyclohexanecarboxylate
-
 Corynebacterium cyclohexanicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.226 27600
-
 SDS-PAGE Corynebacterium cyclohexanicum
1.1.1.226 53600
-
 gel filtration Corynebacterium cyclohexanicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.226 trans-4-hydroxycyclohexanecarboxylate + NAD+ Corynebacterium cyclohexanicum
-
 4-oxocyclohexanecarboxylate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.226 Corynebacterium cyclohexanicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.226
-
 Corynebacterium cyclohexanicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.226 1.48
-
 crude extract, NADH oxidation Corynebacterium cyclohexanicum
1.1.1.226 40
-
 purified enzyme, NAD reduction Corynebacterium cyclohexanicum
1.1.1.226 104.1
-
 purified enzyme, NADH oxidation Corynebacterium cyclohexanicum

Storage Stability

EC Number Storage Stability Organism
1.1.1.226 -80°C Corynebacterium cyclohexanicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.226 trans-4-hydroxycyclohexanecarboxylate + NAD+
-
 Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
 r
1.1.1.226 trans-4-hydroxycyclohexanecarboxylate + NAD+ higher specific activities assayed for reverse reaction Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
 r
1.1.1.226 trans-4-hydroxycyclohexanecarboxylate + NAD+ highly specific for trans isomer Corynebacterium cyclohexanicum 4-oxocyclohexanecarboxylate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.226 dimer 2 * 27600, gel filtration, SDS-PAGE Corynebacterium cyclohexanicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.226 5.8 7.2 NADH oxidation, depending on the buffer system Corynebacterium cyclohexanicum
1.1.1.226 8
-
 NAD reduction Corynebacterium cyclohexanicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.226 6 9
-
 Corynebacterium cyclohexanicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.226 NAD+ very specific, no activity with NADP+ Corynebacterium cyclohexanicum
1.1.1.226 NADH very specific, no activity with NADPH Corynebacterium cyclohexanicum