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Literature summary extracted from

  • Daniel, R.; Boenigk, R.; Gottschalk, G.
    Purification of 1,3-propanediol dehydrogenase from Citrobacter freundii and cloning, sequencing, and overexpression of the corresponding gene in Escherichia coli (1995), J. Bacteriol., 177, 2151-2156.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 overexpression in Escherichia coli Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.202 0.033
-
 NADH
-
 Citrobacter freundii
1.1.1.202 0.3
-
 NAD+
-
 Citrobacter freundii
1.1.1.202 1.25
-
 Propane-1,3-diol
-
 Citrobacter freundii
1.1.1.202 11
-
 propionaldehyde
-
 Citrobacter freundii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.202 Fe2+
-
 Citrobacter freundii
1.1.1.202 Mn2+
-
 Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.202 43400
-
 8 * 43400, SDS-PAGE Citrobacter freundii
1.1.1.202 331000
-
 gel filtration, sedimentation coefficient Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.202 propane-1,3-diol + NAD+ Citrobacter freundii
-
 3-hydroxypropanal + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Citrobacter freundii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.202
-
 Citrobacter freundii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.202 90.9
-
-
 Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.202 1,4-butanediol + NAD+
-
 Citrobacter freundii 4-hydroxybutanal + NADH
-
 r
1.1.1.202 1-butanol + NAD+
-
 Citrobacter freundii NADH + butanal
-
 r
1.1.1.202 1-propanol + NAD+
-
 Citrobacter freundii NADH + propanal
-
 r
1.1.1.202 2,3-butanediol + NAD+
-
 Citrobacter freundii ? + NADH
-
 r
1.1.1.202 2-butanol + NAD+
-
 Citrobacter freundii NADH + butanone
-
 ?
1.1.1.202 ethanol + NAD+
-
 Citrobacter freundii NADH + ethanal
-
 r
1.1.1.202 ethylene glycol + NAD+
-
 Citrobacter freundii NADH + ?
-
 r
1.1.1.202 glycerol + NAD+
-
 Citrobacter freundii glyceraldehyde + NADH + H+
-
 r
1.1.1.202 propane-1,2-diol + NAD+
-
 Citrobacter freundii 2-hydroxypropanal + NADH
-
 r
1.1.1.202 propane-1,3-diol + NAD+
-
 Citrobacter freundii 3-hydroxypropanal + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.202 octamer 8 * 43400, SDS-PAGE Citrobacter freundii