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Literature summary extracted from

  • Dalkin, K.; Edwards, R.; Edington, B.; Dixon, R.A.
    Stress responses in alfalfa (Medicago sativa L.). I. Induction of phenylpropanoid biosynthesis and hydrolytic enzymes in elicitor-treated cell suspension cultures (1990), Plant Physiol., 92, 440-446.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.150 dithioerythritol 10-20 mM, up to 39% inhibition Medicago sativa
2.1.1.150 dithiothreitol 10-20 mM, up to 39% inhibition Medicago sativa

General Stability

EC Number General Stability Organism
2.1.1.150 stabilized in the presence of EDTA and thiol-containing reagents Medicago sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.150 additional information enzyme activity was not affected by inclusion of 5 mM Mn2+, Mg2+, or EDTA in the assay mixture Medicago sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.150 41000
-
 1 * 41000, SDS-PAGE Medicago sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.194 coniferyl alcohol + NADP+ Medicago sativa
-
 coniferyl aldehyde + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.91 Medicago sativa
-
 alfalfa
-
1.1.1.194 Medicago sativa
-
-
-
2.1.1.150 Medicago sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.150
-
 Medicago sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.150 cell suspension culture
-
 Medicago sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.91 additional information
-
 specific activity is 11.99 U/mg protein Medicago sativa
1.1.1.91 additional information
-
 coniferyl alcohol dehydrogenase is involved in the biosynthesis of wallbound phenolics and lignin Medicago sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.194 coniferyl alcohol + NADP+
-
 Medicago sativa coniferyl aldehyde + NADPH + H+
-
 ?
2.1.1.150 additional information IOMT II does not catalyse the methylation of apigenin, kaempferol, flavonoids mono-hydroxylated in the 2’,3,4’,5, or 7 positions, flavanones such as naringenin or coumestans such as coumestrol Medicago sativa ?
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 5,7,4'-trihydroxyisoflavone i.e. genistein. 50% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 5,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 6,7,4'-trihydroxyisoflavone i.e. glycitein. 178% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 6,4'-dihydroxy-7-methoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,2'-dihydroxy-4'-methoxyisoflavone i.e. 2'-hydroxyformononetin. 55% of the activity with 7,4'-dihydroxyisoflavone (i.e. daidzein) Medicago sativa S-adenosyl-L-homocysteine + 2-hydroxy-7,4'-dimethoxyisoflavone
-
 ?
2.1.1.150 S-adenosyl-L-methionine + 7,4'-dihydroxyisoflavone i.e. daidzein Medicago sativa S-adenosyl-L-homocysteine + 4'-hydroxy-7-methoxyisoflavone i.e. isoformononetin ?

Subunits

EC Number Subunits Comment Organism
2.1.1.150 monomer 1 * 41000, SDS-PAGE Medicago sativa

Synonyms

EC Number Synonyms Comment Organism
2.1.1.150 IOMT II
-
 Medicago sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.150 8.5
-
 Tris buffer Medicago sativa
2.1.1.150 8.7
-
 glycine-NaOH buffer Medicago sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.194 NADP+
-
 Medicago sativa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.150 Medicago sativa IOMT IIB, chromatography
-
 4.3
2.1.1.150 Medicago sativa IOMT IIA, chromatography
-
 4.9

Expression

EC Number Organism Comment Expression
2.1.1.150 Medicago sativa treatment of alfalfa cell suspension cultures with elicitor preparations from Saccharomyces cerevisiae or from cell walls of Colletotrichum lindemuthianum results in a ca 200fold induction of isoflavone 0-methyltransferase activity up