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Literature summary extracted from
- Bacillus subtiltis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli (1991), Gene, 108, 121-125.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.18 | expression in Escherichia coli | Bacillus subtilis |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.18 | 30000 | - |
x * 30000, SDS-PAGE | Bacillus subtilis |
| 1.1.1.18 | 160000 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.18 | myo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.18 | Bacillus subtilis | P26935 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.18 | 90% homogeneity, recombinant enzyme | Bacillus subtilis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.18 | cell culture | - |
Bacillus subtilis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.18 | 12 | - |
overepression in Escherichia coli | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.18 | D-glucose + NAD+ | 9% of the activity compared to myo-inositol as substrate | Bacillus subtilis | D-gluconate + NADH | - |
? | |
| 1.1.1.18 | D-xylose + NAD+ | 7% of the activity compared to myo-inositol as substrate | Bacillus subtilis | ? + NADH | - |
? | |
| 1.1.1.18 | myo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.18 | ? | x * 30000, SDS-PAGE | Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.18 | NAD+ | can not be replaced by NADP+ | Bacillus subtilis | |
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Release 2023.1 (January 2023)
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