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Literature summary extracted from

  • Kiser, R.C.; Niehaus, W.G.
    Purification and kinetic characterization of mannitol-1-phosphate dehydrogenase from Aspergillus niger (1981), Arch. Biochem. Biophys., 211, 613-621.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.17 adenosine diphosphoribose competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate Aspergillus niger
1.1.1.17 D-fructose 6-phosphate competitive inhibition with respect to D-mannitol 1-phosphate Aspergillus niger
1.1.1.17 D-glucose 6-phosphate competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH Aspergillus niger
1.1.1.17 D-mannitol 1-phosphate competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH Aspergillus niger
1.1.1.17 NAD+ competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate Aspergillus niger
1.1.1.17 NADH competitive inhibition with respect to NAD+ Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.17 0.005
-
NADH cosubstrate: D-fructose 6-phosphate Aspergillus niger
1.1.1.17 0.038
-
D-mannitol 1-phosphate cosubstrate: NAD+ Aspergillus niger
1.1.1.17 0.083
-
NAD+ cosubstrate: D-mannnitol 1-phosphate Aspergillus niger
1.1.1.17 0.54
-
D-fructose 6-phosphate cosubstrate: NADH Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.17 intracellular
-
Aspergillus niger 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.17 22000
-
2 * 22000, SDS-PAGE Aspergillus niger
1.1.1.17 40000
-
gel filtration Aspergillus niger

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + NAD+ Aspergillus niger highly specific for both substrates D-fructose 6-phosphate + NADH + H+
-
r
1.1.1.17 D-mannitol 1-phosphate + NAD+ Aspergillus niger random bi-bi kinetic with two dead-end complexes D-fructose 6-phosphate + NADH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.17 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.17 to homogeneity, chromatography techniques Aspergillus niger

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.17 cell culture
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.17 150
-
-
Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.17 D-mannitol 1-phosphate + acetylpyridine adenine dinucleotide 50% of the activity compared to NAD+ Aspergillus niger D-fructose 6-phosphate + ?
-
?
1.1.1.17 D-mannitol 1-phosphate + NAD+ highly specific for both substrates Aspergillus niger D-fructose 6-phosphate + NADH + H+
-
r
1.1.1.17 D-mannitol 1-phosphate + NAD+ random bi-bi kinetic with two dead-end complexes Aspergillus niger D-fructose 6-phosphate + NADH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.17 dimer 2 * 22000, SDS-PAGE Aspergillus niger

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.17 5.5 10 more than 95% of its original activity Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.17 NAD+ strictly specific Aspergillus niger
1.1.1.17 NADH
-
Aspergillus niger