EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.17 | adenosine diphosphoribose | competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate | Aspergillus niger | |
1.1.1.17 | D-fructose 6-phosphate | competitive inhibition with respect to D-mannitol 1-phosphate | Aspergillus niger | |
1.1.1.17 | D-glucose 6-phosphate | competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH | Aspergillus niger | |
1.1.1.17 | D-mannitol 1-phosphate | competitive inhibition with respect to D-fructose 6-phosphate, noncompetitive inhibition with respect to NADH | Aspergillus niger | |
1.1.1.17 | NAD+ | competitive inhibition with respect to NADH, noncompetitive inhibition with respect to D-fructose 6-phosphate | Aspergillus niger | |
1.1.1.17 | NADH | competitive inhibition with respect to NAD+ | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.17 | 0.005 | - |
NADH | cosubstrate: D-fructose 6-phosphate | Aspergillus niger | |
1.1.1.17 | 0.038 | - |
D-mannitol 1-phosphate | cosubstrate: NAD+ | Aspergillus niger | |
1.1.1.17 | 0.083 | - |
NAD+ | cosubstrate: D-mannnitol 1-phosphate | Aspergillus niger | |
1.1.1.17 | 0.54 | - |
D-fructose 6-phosphate | cosubstrate: NADH | Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.17 | intracellular | - |
Aspergillus niger | 5622 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 22000 | - |
2 * 22000, SDS-PAGE | Aspergillus niger |
1.1.1.17 | 40000 | - |
gel filtration | Aspergillus niger |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | Aspergillus niger | highly specific for both substrates | D-fructose 6-phosphate + NADH + H+ | - |
r | |
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | Aspergillus niger | random bi-bi kinetic with two dead-end complexes | D-fructose 6-phosphate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.17 | Aspergillus niger | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.17 | to homogeneity, chromatography techniques | Aspergillus niger |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.17 | cell culture | - |
Aspergillus niger | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 150 | - |
- |
Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.17 | D-mannitol 1-phosphate + acetylpyridine adenine dinucleotide | 50% of the activity compared to NAD+ | Aspergillus niger | D-fructose 6-phosphate + ? | - |
? | |
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | highly specific for both substrates | Aspergillus niger | D-fructose 6-phosphate + NADH + H+ | - |
r | |
1.1.1.17 | D-mannitol 1-phosphate + NAD+ | random bi-bi kinetic with two dead-end complexes | Aspergillus niger | D-fructose 6-phosphate + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.17 | dimer | 2 * 22000, SDS-PAGE | Aspergillus niger |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.17 | 5.5 | 10 | more than 95% of its original activity | Aspergillus niger |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.17 | NAD+ | strictly specific | Aspergillus niger | |
1.1.1.17 | NADH | - |
Aspergillus niger |