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Literature summary extracted from

  • Schneider, K.H.; Jäkel, G.; Hoffmann, R.; Giffhorn, F.
    Enzyme evolution in Rhodobacter sphaeroides: Selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme (1995), Microbiology, 141, 1865-1873.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.406 sucrose stabilizes enzyme preparation Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.406 2,2'-dipyridyl 13% inhibition at 1 mM, restored by addition of 2 mM MgCl2 Cereibacter sphaeroides
1.1.1.406 EDTA 68% inhibition at 1 mM, restored by addition of 2 mM MgCl2 Cereibacter sphaeroides
1.1.1.406 FeSO4
-
 Cereibacter sphaeroides
1.1.1.406 ZnSO4
-
 Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.406 0.004
-
 NADH
-
 Cereibacter sphaeroides
1.1.1.406 0.012
-
 NAD+
-
 Cereibacter sphaeroides
1.1.1.406 0.2
-
 1,2-hexanediol
-
 Cereibacter sphaeroides
1.1.1.406 48
-
 dihydroxyacetone
-
 Cereibacter sphaeroides
1.1.1.406 62
-
 acetoin
-
 Cereibacter sphaeroides
1.1.1.406 85
-
 D-threitol
-
 Cereibacter sphaeroides
1.1.1.406 144
-
 L-erythrulose
-
 Cereibacter sphaeroides
1.1.1.406 240
-
 galactitol
-
 Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.406 Co2+ 21% activation at 1 mM Cereibacter sphaeroides
1.1.1.406 Mg2+ 1 mM required for optimal activity Cereibacter sphaeroides
1.1.1.406 Mn2+ 1 mM required for optimal activity Cereibacter sphaeroides
1.1.1.406 Ni2+ 25% activation at 1 mM Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.406 28000
-
 4 * 28000, SDS-PAGE Cereibacter sphaeroides
1.1.1.406 110000
-
 sucrose density gradient centrifugation Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.406 D-arabitol + NAD+ Cereibacter sphaeroides
-
 ? + NADH
-
 ?
1.1.1.406 D-ribulose + NADH Cereibacter sphaeroides
-
 ? + NAD+
-
 ?
1.1.1.406 D-threitol + NAD+ Cereibacter sphaeroides best substrate tested ? + NADH
-
 ?
1.1.1.406 D-xylulose + NADH Cereibacter sphaeroides
-
 ? + NAD+
-
 ?
1.1.1.406 galactitol + NAD+ Cereibacter sphaeroides
-
 L-tagatose + NADH
-
 r
1.1.1.406 L-erythrulose + NADH Cereibacter sphaeroides
-
 ? + NAD+
-
 ?
1.1.1.406 L-glucitol + NAD+ Cereibacter sphaeroides
-
 ? + NADH
-
 ?
1.1.1.406 xylitol + NAD+ Cereibacter sphaeroides high activity L-xylulose + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.406 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.406 to homogeneity, chromatography steps Cereibacter sphaeroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.406 cell culture
-
 Cereibacter sphaeroides
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.406 4°C, 20 mM Bis-Tris, pH 6.5, 1 mM MgCl2, 30% sucrose, stable for 3 months Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.406 (2R)-butanol + NAD+
-
 Cereibacter sphaeroides 2-butanone + NADH
-
 ?
1.1.1.406 (2S)-butanol + NAD+
-
 Cereibacter sphaeroides 2-butanone + NADH
-
 ?
1.1.1.406 (2S,3S)-butanediol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 1,2-butanediol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 1,2-hexanediol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 1,2-pentanediol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 1,2-propanediol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 2-hexanol + NAD+
-
 Cereibacter sphaeroides 2-hexanone + NADH
-
 ?
1.1.1.406 2-propanol + NAD+
-
 Cereibacter sphaeroides acetone + NADH
-
 ?
1.1.1.406 acetoin + NADH
-
 Cereibacter sphaeroides ? + NAD+
-
 ?
1.1.1.406 D-arabitol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 D-ribulose + NADH
-
 Cereibacter sphaeroides ? + NAD+
-
 ?
1.1.1.406 D-threitol + NAD+ best substrate tested Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 D-xylulose + NADH
-
 Cereibacter sphaeroides ? + NAD+
-
 ?
1.1.1.406 dihydroxyacetone + NADH
-
 Cereibacter sphaeroides ? + NAD+
-
 ?
1.1.1.406 galactitol + NAD+
-
 Cereibacter sphaeroides L-tagatose + NADH
-
 r
1.1.1.406 glycerol + NAD+
-
 Cereibacter sphaeroides DL-glyceraldehyde + NADH
-
 r
1.1.1.406 L-erythrulose + NADH
-
 Cereibacter sphaeroides ? + NAD+
-
 ?
1.1.1.406 L-glucitol + NAD+
-
 Cereibacter sphaeroides ? + NADH
-
 ?
1.1.1.406 xylitol + NAD+ high activity Cereibacter sphaeroides L-xylulose + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.406 tetramer 4 * 28000, SDS-PAGE Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.406 37
-
 50% loss of activity after 8 days Cereibacter sphaeroides
1.1.1.406 50
-
 50% loss of activity after 12 min Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.406 4
-
 reduction of acetoin Cereibacter sphaeroides
1.1.1.406 10.5
-
 1,2-hexanediol oxidation Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.406 6.5
-
 63% of the reduction activity Cereibacter sphaeroides
1.1.1.406 9
-
 81% of the oxidation activity Cereibacter sphaeroides

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.406 NAD+ strictly specific Cereibacter sphaeroides
1.1.1.406 NADH strictly specific Cereibacter sphaeroides