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Literature summary extracted from

  • Kerenyi, G.; Szentirmai, A.; Natonek, M.
    Properties of delta5-3beta-hydroxysteroid oxidoreductase isolated from Streptomyces griseocarneus (1975), Acta Microbiol. Acad. Sci. Hung., 22, 487-496.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 pregnenolone + NAD(P)+ Streptomyces griseocarneus
-
 pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?
1.1.3.6 cholesterol + O2 Streptomyces griseocarneus
-
 cholest-5-en-3-one + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Streptomyces griseocarneus
-
-
-
1.1.3.6 Streptomyces griseocarneus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145 partial Streptomyces griseocarneus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.6 cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Streptomyces griseocarneus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
 Streptomyces griseocarneus 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
 ?
1.1.1.145 additional information specific for 3beta-OH group in steroids with trans configuration of A/B rings and DELTA5-double bond Streptomyces griseocarneus ?
-
 ?
1.1.1.145 pregnenolone + NAD(P)+
-
 Streptomyces griseocarneus pregn-5-en-3,20-dione + NAD(P)H + H+
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces griseocarneus cholest-4-en-3-one + H2O2
-
 ?
1.1.3.6 cholesterol + O2
-
 Streptomyces griseocarneus cholest-5-en-3-one + H2O2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.145 45
-
-
 Streptomyces griseocarneus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.145 7.5
-
-
 Streptomyces griseocarneus