EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.187 | GDP-alpha-D-mannose | competitive inhibitor | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.187 | 0.025 | - |
GDP-4-dehydro-6-deoxy-D-mannose | with NADPH as reductant | Pseudomonas sp. | |
1.1.1.187 | 0.027 | - |
NADH | - |
Pseudomonas sp. | |
1.1.1.187 | 0.028 | - |
NADPH | - |
Pseudomonas sp. | |
1.1.1.187 | 0.05 | - |
GDP-4-dehydro-6-deoxy-D-mannose | with NADH as reductant | Pseudomonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.187 | 50000 | 70000 | gel filtration | Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.187 | GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | Pseudomonas sp. | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | GDP-6-deoxy-D-mannose + NAD(P)+ | - |
? | |
1.1.1.187 | GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | Pseudomonas sp. | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | GDP-D-talometylose + NAD(P)+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.187 | Pseudomonas sp. | - |
soil bacterium GS, ATCC 19241 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.1.1.187 | DTT reduces instability at room temperature from 51% to 35% | Pseudomonas sp. |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.187 | to homogeneity, fractionation-, chromatography-techniques | Pseudomonas sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.187 | cell culture | - |
Pseudomonas sp. | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.187 | 1.98 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.187 | -20°C, half-life: 5 months | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.187 | GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | Pseudomonas sp. | GDP-6-deoxy-D-mannose + NAD(P)+ | - |
? | |
1.1.1.187 | GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H | lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function | Pseudomonas sp. | GDP-D-talometylose + NAD(P)+ | - |
? | |
1.1.1.187 | GDP-4-dehydro-alpha-D-rhamnose + NADH + H+ | - |
Pseudomonas sp. | GDP-alpha-D-rhamnose + NAD+ | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.187 | GDP KR reductase | - |
Pseudomonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.187 | 56 | - |
loss of activity in 5 min | Pseudomonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.187 | 6 | 7 | with NADPH as reductant | Pseudomonas sp. |
1.1.1.187 | 8 | - |
with NADH as reductant | Pseudomonas sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.187 | NADH | works equally well compared to NADPH | Pseudomonas sp. | |
1.1.1.187 | NADPH | - |
Pseudomonas sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.187 | 0.72 | - |
GDP-mannose | competitive inhibition | Pseudomonas sp. |