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Literature summary extracted from

  • Winkler, N.W.; Markovitz, A.
    Guanosine diphosphate-4-keto-D-rhamnose reductase. A non-stereoselective enzyme (1971), J. Biol. Chem., 246, 5868-5876.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.187 GDP-alpha-D-mannose competitive inhibitor Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.187 0.025
-
 GDP-4-dehydro-6-deoxy-D-mannose with NADPH as reductant Pseudomonas sp.
1.1.1.187 0.027
-
 NADH
-
 Pseudomonas sp.
1.1.1.187 0.028
-
 NADPH
-
 Pseudomonas sp.
1.1.1.187 0.05
-
 GDP-4-dehydro-6-deoxy-D-mannose with NADH as reductant Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.187 50000 70000 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.187 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H Pseudomonas sp. lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function GDP-6-deoxy-D-mannose + NAD(P)+
-
 ?
1.1.1.187 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H Pseudomonas sp. lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function GDP-D-talometylose + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.187 Pseudomonas sp.
-
 soil bacterium GS, ATCC 19241
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.187 DTT reduces instability at room temperature from 51% to 35% Pseudomonas sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.187 to homogeneity, fractionation-, chromatography-techniques Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.187 cell culture
-
 Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.187 1.98
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.1.1.187 -20°C, half-life: 5 months Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.187 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function Pseudomonas sp. GDP-6-deoxy-D-mannose + NAD(P)+
-
 ?
1.1.1.187 GDP-4-dehydro-6-deoxy-D-mannose + NAD(P)H lacks precise stereoselectivity with respect to its hydrogen receptor carbonyl function Pseudomonas sp. GDP-D-talometylose + NAD(P)+
-
 ?
1.1.1.187 GDP-4-dehydro-alpha-D-rhamnose + NADH + H+
-
 Pseudomonas sp. GDP-alpha-D-rhamnose + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.187 GDP KR reductase
-
 Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.187 56
-
 loss of activity in 5 min Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.187 6 7 with NADPH as reductant Pseudomonas sp.
1.1.1.187 8
-
 with NADH as reductant Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.187 NADH works equally well compared to NADPH Pseudomonas sp.
1.1.1.187 NADPH
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.187 0.72
-
 GDP-mannose competitive inhibition Pseudomonas sp.