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Literature summary extracted from

  • Benedetti, P.; Baldi, M.I.; Mattoccia, E.; Tocchini-Valentini, G.P.
    Purification and characterization of Xenopus laevis topoisomerase II (1983), EMBO J., 2(8), 1303-1308.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.6.2.2 spermidine stimulates catenation reaction Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 nucleus
-
 Xenopus laevis 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 K+ catenation reaction is very sensitive to ionic strength, being optimal in the range 20-30 mM KCl or 20-50 mM NH4Cl Xenopus laevis
5.6.2.2 Mg2+ required Xenopus laevis
5.6.2.2 NH4Cl catenation reaction is very sensitive to ionic strength, being optimal in the range 20-30 mM KCl or 20-50 mM NH4Cl Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 175000
-
 sucrose density gradient centrifugation Xenopus laevis

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2
-
 Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 oocyte
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.2 additional information
-
-
 Xenopus laevis

Storage Stability

EC Number Storage Stability Organism
5.6.2.2 -70°C, partially purified enzyme is stable for at least 3 months. Purified enzyme loses 50% of its activity after 3 d Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information ATP hydrolysis Xenopus laevis ?
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Xenopus laevis monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Xenopus laevis catenated DNA networks + ADP + phosphate in presence of spermine circular DNA is catenated to dimers, trimer and a network ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Xenopus laevis relaxed DNA + ADP + phosphate
-
 ?