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Literature summary extracted from

  • Knudsen, B.R.; Straub, T.; Boege, F.
    Separation and functional analysis of eukaryotic DNA topoisomerases by chromatography and electrophoresis (1996), J. Chromatogr., 684, 307-321.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.6.2.1 pharmacology important cellular target in the treatment of human cancer eukaryota
5.6.2.2 pharmacology important cellular target in the treatment of human cancers eukaryota

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.1 overexpressed in yeast Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.1 nucleus
-
 Homo sapiens 5634
-
5.6.2.1 nucleus
-
 eukaryota 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.6.2.1 additional information Homo sapiens
-
 ?
-
 ?
5.6.2.1 additional information eukaryota controls the DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps, plays a crucial role in the regulation of the physiological function of the genome ?
-
 ?
5.6.2.2 additional information Bacteria
-
 ?
-
 ?
5.6.2.2 additional information eukaryota controls DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through transient gaps. Plays a crucial function in the regulation of the physiological function of the genome ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 eukaryota
-
-
-
5.6.2.1 Homo sapiens
-
-
-
5.6.2.2 Bacteria
-
-
-
5.6.2.2 eukaryota
-
-
-
5.6.2.2 Mammalia
-
 two structurally related isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1 enzyme overexpressed in yeast Homo sapiens
5.6.2.1 strategy for purifying eukaryotic type I topoisomerases eukaryota
5.6.2.2 general purification strategy eukaryota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 additional information expression of topoisomerase II is proliferation-dependent and alters during the cell cycle eukaryota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 additional information
-
 Homo sapiens ?
-
 ?
5.6.2.1 additional information controls the DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through the transient gaps, plays a crucial role in the regulation of the physiological function of the genome eukaryota ?
-
 ?
5.6.2.1 supercoiled DNA relaxation eukaryota relaxed closed circular DNA
-
 ?
5.6.2.1 supercoiled DNA relaxation Homo sapiens relaxed closed circular DNA
-
 ?
5.6.2.2 additional information
-
 Bacteria ?
-
 ?
5.6.2.2 additional information controls DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through transient gaps. Plays a crucial function in the regulation of the physiological function of the genome eukaryota ?
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Mammalia monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation Bacteria monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 network of DNA rings + ATP + H2O decatenation eukaryota monomeric DNA circles + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Mammalia catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation Bacteria catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O catenation eukaryota catenated DNA networks + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Mammalia relaxed DNA + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation Bacteria relaxed DNA + ADP + phosphate
-
 ?
5.6.2.2 supercoiled DNA + ATP + H2O relaxation eukaryota relaxed DNA + ADP + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 dimer dimer of identical subunits eukaryota