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Literature summary extracted from

  • Wise, M.L.; Savage, T.J.; Katahira, E.; Croteau, R.
    Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase (1998), J. Biol. Chem., 273, 14891-14899.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.108 expression in Escherichia coli Salvia officinalis
4.2.3.110 expression in Escherichia coli Salvia officinalis
4.2.3.114 expression in Escherichia coli Salvia officinalis
5.5.1.8 expression in Escherichia coli Salvia officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.108 0.007
-
 geranyl diphosphate pH 7.1, 31°C Salvia officinalis
4.2.3.110 0.0074
-
 geranyl diphosphate pH 7.1, 31°C Salvia officinalis
4.2.3.114 0.0074
-
 geranyl diphosphate pH 7.1, 31°C Salvia officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.108 chloroplast
-
 Salvia officinalis 9507
-
4.2.3.110 chloroplast
-
 Salvia officinalis 9507
-
4.2.3.114 chloroplast
-
 Salvia officinalis 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.108 69400
-
 x * 69400, calculated Salvia officinalis
4.2.3.108 72000
-
 gel filtration Salvia officinalis
4.2.3.110 68490
-
 x * 68490, calculated Salvia officinalis
4.2.3.114 68490
-
 x * 68490, calculated Salvia officinalis

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.108 Salvia officinalis O81191
-
-
4.2.3.110 Salvia officinalis O81193
-
-
4.2.3.114 Salvia officinalis O81193
-
-
5.5.1.8 Salvia officinalis O81192
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.110 geranyl diphosphate = (+)-sabinene + diphosphate major products sabinene and gamma-terpinene are formed by a cyclization mechanism involving a 1,2-hydride shift in the a-terpinyl cation intermediate Salvia officinalis
a
4.2.3.114 geranyl diphosphate = gamma-terpinene + diphosphate major products sabinene and gamma-terpinene are formed by a cyclization mechanism involving a 1,2-hydride shift in the alpha-terpinyl cation intermediate Salvia officinalis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.108 geranyl diphosphate + H2O
-
 Salvia officinalis 1,8-cineole + diphosphate 79% 1,8-cineole plus 20% of a mixture of (+)- and (-)-alpha-pinene, (+)- and (-)-beta-pinene, myrcene and (+)-sabinene ?
4.2.3.110 geranyl diphosphate + H2O
-
 Salvia officinalis (+)-sabinene + diphosphate 63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene ?
4.2.3.114 geranyl diphosphate + H2O
-
 Salvia officinalis gamma-terpinene + diphosphate 63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene ?
5.5.1.8 Geranyl diphosphate
-
 Salvia officinalis (+)-Bornyl-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.108 monomer x * 69400, calculated Salvia officinalis
4.2.3.110 ? x * 68490, calculated Salvia officinalis
4.2.3.114 ? x * 68490, calculated Salvia officinalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.3.108 Salvia officinalis calculated
-
 5.8
4.2.3.110 Salvia officinalis calculated
-
 5.2
4.2.3.114 Salvia officinalis calculated
-
 5.2