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Literature summary extracted from

  • Mishina, M.; Kamiryo, T.; Numa, S.
    Acetyl-CoA carboxylase from Candida lipolytica. EC 6.4.1.2 Acetyl-CoA:carbon-dioxide ligase (ADP-forming) (1981), Methods Enzymol., 71, 37-44.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 citrate no effect Yarrowia lipolytica
6.4.1.2 Polyethylene glycols of MW 7500 or 3000, activate Yarrowia lipolytica

General Stability

EC Number General Stability Organism
6.4.1.2 extremely labile without stabilizer, e.g. glycerol Yarrowia lipolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 acetone
-
 Yarrowia lipolytica
6.4.1.2 dioxane
-
 Yarrowia lipolytica
6.4.1.2 ethanol
-
 Yarrowia lipolytica
6.4.1.2 ethylene glycol monomethyl ether
-
 Yarrowia lipolytica
6.4.1.2 glycerol
-
 Yarrowia lipolytica
6.4.1.2 tetrahydrofuran
-
 Yarrowia lipolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.07
-
 ATP ATP Yarrowia lipolytica
6.4.1.2 0.26
-
 acetyl-CoA
-
 Yarrowia lipolytica
6.4.1.2 3.7
-
 HCO3-
-
 Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 230000
-
 x * 230000, SDS-PAGE Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Yarrowia lipolytica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Yarrowia lipolytica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 additional information
-
-
 Yarrowia lipolytica

Storage Stability

EC Number Storage Stability Organism
6.4.1.2 -70°C, 20% glycerol, stable for at least 6 months Yarrowia lipolytica
6.4.1.2 0°C, 20% glycerol, 24 h, stable Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Yarrowia lipolytica ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + hydrogencarbonate ATP in form of MgATP2- Yarrowia lipolytica ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- 13% of the activity relative to acetyl-CoA Yarrowia lipolytica ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 ? x * 230000, SDS-PAGE Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 7.9 8.1
-
 Yarrowia lipolytica

Cofactor

EC Number Cofactor Comment Organism Structure
6.4.1.2 biotin contains 1 mol of biotin per 263000 g of protein Yarrowia lipolytica