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Literature summary extracted from

  • Finlayson, S.A.; Dennis, D.T.
    Acetyl-coenzyme A carboxylase from the developing endosperm of Ricinus communis. I. Isolation and characterization (1983), Arch. Biochem. Biophys., 225, 576-585.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.4.1.2 high concentrations of enzyme are required for stability as well as the presence of DTT, glycerol, HCO3-, Triton X-100, and polyvinylpyrrolidone Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 diphosphate
-
 Ricinus communis
6.4.1.2 glyoxylate
-
 Ricinus communis
6.4.1.2 NADH
-
 Ricinus communis
6.4.1.2 NADPH
-
 Ricinus communis
6.4.1.2 oxaloacetate
-
 Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.05
-
 acetyl-CoA
-
 Ricinus communis
6.4.1.2 0.1
-
 ATP
-
 Ricinus communis
6.4.1.2 3
-
 HCO3-
-
 Ricinus communis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.4.1.2 microsome
-
 Ricinus communis
-
-
6.4.1.2 plastid membrane
-
 Ricinus communis 42170
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Cs+ small activating effect, depends on the degree of saturation of the reaction by HCO3- Ricinus communis
6.4.1.2 K+ small activating effect, depends on the degree of saturation of the reaction by HCO3- Ricinus communis
6.4.1.2 Mg2+ both MgATP2- and free Mg2+ required. Maximal activation at 5 mM Mg2+ Ricinus communis
6.4.1.2 Mg2+ Mg2+ or Mn2+ essential for activity Ricinus communis
6.4.1.2 Mn2+ Mn2+ or Mg2+ essential for activity. Maximal activation at 1.5 mM Mn2+ Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 528000
-
 glycerol density gradient centrifugation Ricinus communis

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Ricinus communis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 seed endosperm Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 2.052
-
-
 Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Ricinus communis ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 UTP + acetyl-CoA + HCO3- at 2.5% of the activity relative to ATP Ricinus communis UDP + phosphate + malonyl-CoA
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.4.1.2 24 28 transition point Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8
-
-
 Ricinus communis