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Literature summary extracted from

  • Nikolau, B.J.; Hawke, J.C.
    Purification and characterization of maize leaf acetyl-coenzyme A carboxylase (1984), Arch. Biochem. Biophys., 228, 86-96.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ADP
-
Zea mays
6.4.1.2 AMP slight Zea mays
6.4.1.2 Li+
-
Zea mays
6.4.1.2 malonyl-CoA
-
Zea mays
6.4.1.2 additional information addition of cations in presence of Mg2+ inhibits Zea mays
6.4.1.2 oxaloacetate weak Zea mays
6.4.1.2 phosphoenolpyruvate weak Zea mays
6.4.1.2 propionyl-CoA below 0.1 mM almost as effective as substrate as acetyl-CoA, inhibitory at higher concentrations Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.11
-
acetyl-CoA
-
Zea mays
6.4.1.2 1.4
-
HCO3-
-
Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Cs+ greater activation at 1 mM HCO3- than at 10 mM HCO3- Zea mays
6.4.1.2 K+ greatest activation at 12-15 mM K+, at HCO3- concentration of 0.18 mM, 1 mM, and 10 mM Zea mays
6.4.1.2 Mg2+ maximal activity at 3 mM Mg2+ with 1 mM ATP and at 4 mM Mg2+ with 3 mM ATP Zea mays
6.4.1.2 Mg2+ free Mg2+ activates Zea mays
6.4.1.2 Mn2+ maximal activity at 1 mM Mn2+ with 1 mM ATP. Maximal activity is 25% of that observed with Mg2+ Zea mays
6.4.1.2 NH4+ greater activation at 1 mM HCO3- than at 10 mM HCO3- Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 500000
-
gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2 acetyl-CoA carboxylase I and acetyl-CoA carboxylase II Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 leaf
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 0.844
-
-
Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Zea mays ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + butyryl-CoA + HCO3- no activity Zea mays ADP + ethylmalonyl-CoA + phosphate
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3- 45% of the activity compared to acetyl-CoA Zea mays ADP + phosphate + methylmalonyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 ?
-
Zea mays
6.4.1.2 octamer 8 * 60000-610000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8.4
-
-
Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.4.1.2 7.4 9.8 50% of maximal activity at pH 7.4 and 9.8 Zea mays