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Literature summary extracted from

  • Blanco, F.; Alana, A.; Llama, M.J.; Serra, J.L.
    Purification and properties of glutamine synthetase from the non-N2-fixing cyanobacterium Phormidium laminosum (1989), J. Bacteriol., 171, 1158-1165.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
6.3.1.2 the presence of Mn2+ or Mg2+ drastically affects the thermostability of transferase activity and biosynthetic activity Phormidium laminosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 ADP biosynthetic activity Phormidium laminosum
6.3.1.2 Ala
-
 Phormidium laminosum
6.3.1.2 AMP biosynthetic activity Phormidium laminosum
6.3.1.2 Asp
-
 Phormidium laminosum
6.3.1.2 Gly
-
 Phormidium laminosum
6.3.1.2 L-Arg biosynthetic activity Phormidium laminosum
6.3.1.2 L-Glu inhibition of transferase activity, no inhibition of biosynthetic activity Phormidium laminosum
6.3.1.2 L-Lys
-
 Phormidium laminosum
6.3.1.2 L-Met
-
 Phormidium laminosum
6.3.1.2 L-methionine sulfoximine
-
 Phormidium laminosum
6.3.1.2 L-Tyr inhibits transferase activity, no inhibition of biosynthetic activity Phormidium laminosum
6.3.1.2 p-hydroxymercuribenzoate biosynthetic activity Phormidium laminosum
6.3.1.2 Ser
-
 Phormidium laminosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Co2+ partially effective as activator of biosynthetic activity and transferase activity, half-maximal activation of biosynthetic reaction at 6.3 mM Phormidium laminosum
6.3.1.2 Cu2+ partially effective as activator of transferase activity Phormidium laminosum
6.3.1.2 Mg2+ essential activator for biosynthetic activity, half-maximal activation at 18 mM. Partially effective as activator of transferase activity Phormidium laminosum
6.3.1.2 Mn2+ required for transferase activity Phormidium laminosum
6.3.1.2 Zn2+ partially effective as activator of biosynthetic activity, half-maximal activation at 6.3 mM Phormidium laminosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 602000
-
 gel filtration Phormidium laminosum

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Phormidium laminosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Phormidium laminosum

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.1.2 ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine ordered three-reactant system with the binding order: NH4+, L-Glu and ATP Phormidium laminosum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 additional information
-
-
 Phormidium laminosum
6.3.1.2 4.4
-
 biosynthetic assay Phormidium laminosum
6.3.1.2 152
-
 gamma-glutamylhydroxamate assay Phormidium laminosum

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 -20°C, stable for several months Phormidium laminosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Phormidium laminosum ADP + phosphate + L-Gln
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer SDS-PAGE of 2-mercaptoethanol-treated enzyme Phormidium laminosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 45
-
 transferase activity Phormidium laminosum
6.3.1.2 55
-
 biosynthetic activity Phormidium laminosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.2
-
-
 Phormidium laminosum
6.3.1.2 7.5
-
 transferase activity Phormidium laminosum