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Literature summary extracted from

  • Hatanaka, M.; Tachiki, T.; Furukawa, R.; Tochikura, T.
    Purification and some properties of glutamine synthetases from Bifidobacteria (1987), Agric. Biol. Chem., 51, 425-433.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
6.3.1.2 adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
6.3.1.2 adenosine more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
6.3.1.2 ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
6.3.1.2 ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
6.3.1.2 ADP more potent inhibition in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
6.3.1.2 Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium bifidum
6.3.1.2 Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium breve
6.3.1.2 Ala more inhibition by L-Ala and glycine in the mixture with Mn2+ than with Mg2+ Bifidobacterium pseudolongum
6.3.1.2 AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium bifidum
6.3.1.2 AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium breve
6.3.1.2 AMP more potent inhibitor in the reaction with Mg2+ than with Mn2+ Bifidobacterium pseudolongum
6.3.1.2 ATP ATP in excess of Mg2+ Bifidobacterium breve
6.3.1.2 ATP ATP in excess of Mg2+ Bifidobacterium pseudolongum
6.3.1.2 Ca2+
-
 Bifidobacterium bifidum
6.3.1.2 Ca2+
-
 Bifidobacterium pseudolongum
6.3.1.2 Cu2+
-
 Bifidobacterium bifidum
6.3.1.2 Cu2+
-
 Bifidobacterium pseudolongum
6.3.1.2 Fe2+
-
 Bifidobacterium bifidum
6.3.1.2 Fe2+
-
 Bifidobacterium pseudolongum
6.3.1.2 Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium bifidum
6.3.1.2 Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium breve
6.3.1.2 Gly more inhibition by L-Ala and Gly in the mixture with Mn2+ than with Mg2+ Bifidobacterium pseudolongum
6.3.1.2 Hg2+
-
 Bifidobacterium bifidum
6.3.1.2 Hg2+
-
 Bifidobacterium pseudolongum
6.3.1.2 Ni2+
-
 Bifidobacterium bifidum
6.3.1.2 Ni2+
-
 Bifidobacterium pseudolongum
6.3.1.2 Pb2+
-
 Bifidobacterium bifidum
6.3.1.2 Zn2+
-
 Bifidobacterium bifidum
6.3.1.2 Zn2+
-
 Bifidobacterium pseudolongum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.56
-
 NH4+ Mg2+-stimulated Bifidobacterium bifidum
6.3.1.2 0.59
-
 NH4+ Mg2+-stimulated Bifidobacterium breve
6.3.1.2 0.69
-
 NH4+ Mn2+-stimulated Bifidobacterium breve
6.3.1.2 0.69
-
 NH4+ NH4+, Mg2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 0.71
-
 NH4+ Mn2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 0.77
-
 NH4+ Mn2+-stimulated Bifidobacterium bifidum
6.3.1.2 1.1
-
 L-Glu Mg2+-stimulated Bifidobacterium bifidum
6.3.1.2 1.3
-
 L-Glu Mn2+-stimulated Bifidobacterium breve
6.3.1.2 1.5
-
 ATP Mg2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 1.7
-
 ATP Mg2+-stimulated Bifidobacterium bifidum
6.3.1.2 2
-
 ATP Mg2+-stimulated or Mn2+-stimulated Bifidobacterium bifidum
6.3.1.2 2.1
-
 L-Glu Mn2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 2.3
-
 ATP Mn2+-stimulated Bifidobacterium breve
6.3.1.2 2.5
-
 ATP Mn2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 18
-
 L-Glu Mg2+-stimulated Bifidobacterium pseudolongum
6.3.1.2 21
-
 L-Glu Mg2+-stimulated Bifidobacterium breve
6.3.1.2 25
-
 L-Glu Mg2+-stimulated Bifidobacterium bifidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Co2+ maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity Bifidobacterium breve
6.3.1.2 Co2+ maximal activity at a ration of ATP to Co2+ of 1.0. The addition of 3 mM Co2+ to the biosynthetic reaction mixtures containing 4 mM Mg2+ results in a 3-7-fold increase in activity Bifidobacterium pseudolongum
6.3.1.2 Co2+ maximal activity when Co2+ is in excess of ATP Bifidobacterium bifidum
6.3.1.2 Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium bifidum
6.3.1.2 Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium breve
6.3.1.2 Co2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective Bifidobacterium pseudolongum
6.3.1.2 Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium bifidum
6.3.1.2 Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium breve
6.3.1.2 Mg2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal when Mg2+ is in excess of ATP Bifidobacterium pseudolongum
6.3.1.2 Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium bifidum
6.3.1.2 Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium breve
6.3.1.2 Mn2+ divalent cation required, Mn2+, Co2+, or Mg2+. Mn2+ is most effective. Activity is maximal at a ratio of ATP to Mn2+ of 1.0 Bifidobacterium pseudolongum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 500000
-
 gel filtration Bifidobacterium bifidum
6.3.1.2 500000
-
 gel filtration Bifidobacterium breve
6.3.1.2 500000
-
 gel filtration Bifidobacterium pseudolongum

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Bifidobacterium bifidum
-
 A
-
6.3.1.2 Bifidobacterium bifidum A
-
 A
-
6.3.1.2 Bifidobacterium breve
-
-
-
6.3.1.2 Bifidobacterium breve 203
-
-
-
6.3.1.2 Bifidobacterium pseudolongum
-
 A
-
6.3.1.2 Bifidobacterium pseudolongum A
-
 A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Bifidobacterium bifidum
6.3.1.2
-
 Bifidobacterium breve
6.3.1.2
-
 Bifidobacterium pseudolongum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 73.2
-
 gamma-glutamyl transferase assay Bifidobacterium breve
6.3.1.2 99.6
-
 gamma-glutamyl transferase assay Bifidobacterium pseudolongum
6.3.1.2 221.3
-
 gamma-glutamyl transferase assay Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium bifidum ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium breve ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium pseudolongum ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium bifidum A ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium breve 203 ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + ethylamine slightly effective Bifidobacterium pseudolongum A ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 13% of the activity relative to NH4+ Bifidobacterium breve ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 59% of the activity relative to NH4+ Bifidobacterium bifidum ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 10% of the activity relative to NH4+ Bifidobacterium pseudolongum ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 59% of the activity relative to NH4+ Bifidobacterium bifidum A ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 13% of the activity relative to NH4+ Bifidobacterium breve 203 ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + hydroxylamine 10% of the activity relative to NH4+ Bifidobacterium pseudolongum A ADP + phosphate + gamma-glutamyl hydroxamate
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium bifidum ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium breve ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium pseudolongum ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium bifidum A ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium breve 203 ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + methylamine slightly effective Bifidobacterium pseudolongum A ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Bifidobacterium bifidum ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Bifidobacterium breve ADP + phosphate + L-Gln
-
 ?
6.3.1.2 ATP + L-Glu + NH4+
-
 Bifidobacterium pseudolongum ADP + phosphate + L-Gln
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium bifidum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium breve gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium pseudolongum gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium bifidum A gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium breve 203 gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Bifidobacterium pseudolongum A gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ no activity Bifidobacterium breve GDP + phosphate + L-Gln
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ no activity Bifidobacterium pseudolongum GDP + phosphate + L-Gln
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ 15% of the activity relative to ATP Bifidobacterium bifidum GDP + phosphate + L-Gln
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ 15% of the activity relative to ATP Bifidobacterium bifidum A GDP + phosphate + L-Gln
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ no activity Bifidobacterium breve 203 GDP + phosphate + L-Gln
-
 ?
6.3.1.2 GTP + L-Glu + NH4+ no activity Bifidobacterium pseudolongum A GDP + phosphate + L-Gln
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 60
-
 pH 5.5, 10 min, 14% loss of activity Bifidobacterium bifidum
6.3.1.2 60
-
 pH 6.0, 10 min, 50% loss of activity Bifidobacterium breve
6.3.1.2 60
-
 pH 6.0, 10 min, 68% loss of activity Bifidobacterium pseudolongum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium bifidum
6.3.1.2 6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium breve
6.3.1.2 6 6.5 biosynthetic reaction, with 30 mM Mg2+ Bifidobacterium pseudolongum
6.3.1.2 6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium bifidum
6.3.1.2 6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium breve
6.3.1.2 6.5
-
 gamma-glutamylhydroxamate forming reaction Bifidobacterium pseudolongum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.1.2 5 6 most stable in this range Bifidobacterium bifidum
6.3.1.2 5.5
-
 60°C, 10 min, 0.1 M acetate buffer, 14% loss of activity Bifidobacterium bifidum
6.3.1.2 6
-
 60°C, 10 min, 58% loss of activity Bifidobacterium pseudolongum
6.3.1.2 6
-
 60°C, 10 min, 0.1 M imidazole-HCl buffer, 50% loss of activity Bifidobacterium breve