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Literature summary extracted from

  • Bednar, R.A.; McCaffrey, C.; Shan, K.
    Introduction of unnatural amino acids into chalcone isomerase (1991), Bioconjug. Chem., 2, 211-216.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.6 additional information production of enzyme derivatives in which the Cys residue has been converted to a series of unnatural amino acids. The results demonstrate that the Cys residue does not function as an acid-base or nucleophilic group during catalysis nor does it play any role in the substrate inhibition Glycine max

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.6 4,2',4'-trihydroxychalcone substrate inhibition Glycine max
5.5.1.6 4,4'-Dihydroxychalcone
-
 Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.0043
-
 4,2',4',6'-Tetrahydroxychalcone methanethiolated enzyme Glycine max
5.5.1.6 0.0063
-
 4,2',4',6'-Tetrahydroxychalcone native enzyme Glycine max
5.5.1.6 0.12
-
 4,2',4'-trihydroxychalcone methanethiolated enzyme Glycine max
5.5.1.6 0.18
-
 4,2',4'-trihydroxychalcone native enzyme Glycine max

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Glycine max
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 2'-Hydroxy-4-methoxychalcone
-
 Glycine max ?
-
 ?
5.5.1.6 4,2',4',6'-Tetrahydroxychalcone
-
 Glycine max 5,7,4'-Trihydroxyflavanone
-
 ?
5.5.1.6 4,2',4'-trihydroxychalcone
-
 Glycine max (2S)-4',7-dihydroxyflavanone
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.6 107
-
 4,2',4'-trihydroxychalcone methanethiolated enzyme Glycine max
5.5.1.6 167
-
 4,2',4'-trihydroxychalcone native enzyme Glycine max
5.5.1.6 700
-
 4,2',4',6'-Tetrahydroxychalcone methanethiolated enzyme Glycine max
5.5.1.6 833
-
 4,2',4',6'-Tetrahydroxychalcone native enzyme Glycine max