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Literature summary extracted from

  • Chakraborty, R.; Chandra, A.L.
    Purification and characterization of a streptomycete collagenase (1986), J. Appl. Bacteriol., 61, 331-337.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.3 2-mercaptoethanol
-
 Streptomyces sp.
3.4.24.3 8-hydroxyquinoline
-
 Streptomyces sp.
3.4.24.3 alpha,alpha-dipyridyl
-
 Streptomyces sp.
3.4.24.3 Cd2+
-
 Streptomyces sp.
3.4.24.3 glutathione reduced Streptomyces sp.
3.4.24.3 Mn2+
-
 Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.3 extracellular
-
 Streptomyces sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.3 Ca2+ activates Streptomyces sp.
3.4.24.3 Mg2+ activates Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.3 75000
-
 1 * 75000, no dissociation into subunits after lithium chloride treatment, SDS-PAGE Streptomyces sp.
3.4.24.3 75000
-
 ? * 75000, SDS-PAGE Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.3 Collagen + H2O Streptomyces sp. degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.3 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.3
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.3 additional information
-
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.3 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg + H2O
-
 Streptomyces sp. 4-phenylazobenzyloxycarbonyl-Pro-Leu + Gly-Pro-Arg
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Streptomyces sp. Hydrolyzed collagen
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Streptomyces sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.3 ? ? * 75000, SDS-PAGE Streptomyces sp.
3.4.24.3 monomer 1 * 75000, no dissociation into subunits after lithium chloride treatment, SDS-PAGE Streptomyces sp.