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Literature summary extracted from

  • Barrett, A.J.; Brown, M.A.
    Chicken liver Pz-peptidase, a thiol-dependent metallo-endopeptidase (1990), Biochem. J., 271, 701-706.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.15 2-mercaptoethanol activates at low concentrations Gallus gallus
3.4.24.15 dithiothreitol activates at low concentrations Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.15 1,10-phenanthroline
-
 Gallus gallus
3.4.24.15 5-cyano-2-nitrothiobenzoate 0.1 mM, 62% inhibition Gallus gallus
3.4.24.15 diethyl dicarbonate reversed by hydroxylamine Gallus gallus
3.4.24.15 EDTA
-
 Gallus gallus
3.4.24.15 Fe2+
-
 Gallus gallus
3.4.24.15 iodoacetate 1 mM, 22% inhibition Gallus gallus
3.4.24.15 N-[1-(RS)-carboxy-3-phenylpropyl]-Ala-Ala-Tyr-p-aminobenzoate
-
 Gallus gallus
3.4.24.15 NEM 1 mM, 82% inhibition Gallus gallus
3.4.24.15 Ni2+
-
 Gallus gallus
3.4.24.15 PCMB 0.1 mM, 100% inhibition Gallus gallus
3.4.24.15 tosyl-Phe-CH3Cl 0.1 mM, 15% inactivation Gallus gallus
3.4.24.15 Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu-Lys i.e. dynorphin-(1-13) Gallus gallus
3.4.24.15 Zn2+ at high concentration Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.15 0.0123
-
 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(N6-(2,4-dinitrophenyl))Lys
-
 Gallus gallus
3.4.24.15 0.0137
-
 2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.15 Ca2+ restores activity after EDTA treatment Gallus gallus
3.4.24.15 Cd2+ restores activity after EDTA treatment Gallus gallus
3.4.24.15 Co2+ restores activity after EDTA treatment Gallus gallus
3.4.24.15 Mn2+ restores activity after EDTA treatment Gallus gallus
3.4.24.15 Zn2+ most effective ion in restoration of the activity after EDTA treatment Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.15 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.15
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.15 liver
-
 Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.15 2,4-dinitrophenyl-Pro-Leu-Gly-Pro-Trp-D-Lys
-
 Gallus gallus ?
-
 ?
3.4.24.15 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-(2,4-ditritrophenyl)Lys
-
 Gallus gallus ?
-
 ?
3.4.24.15 7-methoxycoumarin-3-carboxylyl-Pro-Leu-Gly-Pro-D-(N6-(2,4-dinitrophenyl))Lys + H2O
-
 Gallus gallus ?
-
 ?
3.4.24.15 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O i.e. bradykinin, cleavage site: Phe5-Ser6 Gallus gallus Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
 ?
3.4.24.15 benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
 Gallus gallus benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
 ?
3.4.24.15 additional information no hydrolysis of proteins Gallus gallus ?
-
 ?
3.4.24.15 N-acetyl-Ala-Ala-Ala
-
 Gallus gallus ?
-
 ?
3.4.24.15 N-acetyl-Ala-Ala-Ala-Ala
-
 Gallus gallus N-acetyl-Ala + Ala-Ala-Ala
-
 ?
3.4.24.15 phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg
-
 Gallus gallus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.15 8
-
-
 Gallus gallus