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Literature summary extracted from

  • Agarwal, S.K.; Choudhury, S.D.; lamsal, M.; Khan, M.Y.
    Catalytic and physico-chemical characteristics of goat spleen cathepsin B (1997), Biochem. Mol. Biol. Int., 42, 1215-1226.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 2-mercaptoethanol activates Capra hircus
3.4.22.1 cysteamine activates Capra hircus
3.4.22.1 cysteine activates Capra hircus
3.4.22.1 dithiothreitol activates Capra hircus
3.4.22.1 glutathione activates Capra hircus
3.4.22.1 thioglycerol activates Capra hircus
3.4.22.1 thiol compounds required for full activity Capra hircus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 1-tosylamide-2-phenylethyl chloromethylketone moderate effect Capra hircus
3.4.22.1 antipain
-
 Capra hircus
3.4.22.1 Hg2+
-
 Capra hircus
3.4.22.1 iodoacetamide
-
 Capra hircus
3.4.22.1 iodoacetic acid
-
 Capra hircus
3.4.22.1 L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Capra hircus
3.4.22.1 leupeptin
-
 Capra hircus
3.4.22.1 Mn2+
-
 Capra hircus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.00145
-
 hemoglobin
-
 Capra hircus
3.4.22.1 0.058
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Capra hircus
3.4.22.1 0.262
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin Capra hircus
3.4.22.1 0.685
-
 benzoyl-DL-Arg-p-nitroanilide
-
 Capra hircus
3.4.22.1 2.5
-
 benzoyl-DL-Arg-beta-naphthylamide
-
 Capra hircus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 26000 28000 gel filtration, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Capra hircus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Capra hircus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.1 side-chain modification 6.3% carbohydrate Capra hircus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 spleen
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.1 0.247
-
 benzoyl-DL-Arg-p-nitroanilide as substrate Capra hircus
3.4.22.1 0.562
-
 benzoyl-DL-Arg-beta-naphthylamide Capra hircus
3.4.22.1 0.65
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide Capra hircus
3.4.22.1 1.13
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide Capra hircus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Capra hircus benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O
-
 Capra hircus benzoyl-DL-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Capra hircus benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Capra hircus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 casein + H2O
-
 Capra hircus hydrolyzed casein
-
 ?
3.4.22.1 Hemoglobin + H2O
-
 Capra hircus ?
-
 ?
3.4.22.1 Proteins + H2O
-
 Capra hircus ?
-
 ?