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Literature summary extracted from
- Purification and characterization of cathepsin B from goat brain (1990), J. Biosci., 15, 397-408.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | dithiothreitol | activates | Capra hircus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.1 | 2,2'-dipyridyl disulfide | - |
Capra hircus | |
| 3.4.22.1 | antipain | - |
Capra hircus | |
| 3.4.22.1 | Dimethylsulfoxide | - |
Capra hircus | |
| 3.4.22.1 | Hg2+ | - |
Capra hircus | |
| 3.4.22.1 | iodoacetamide | - |
Capra hircus | |
| 3.4.22.1 | iodoacetic acid | - |
Capra hircus | |
| 3.4.22.1 | L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane | E-64 | Capra hircus | |
| 3.4.22.1 | leupeptin | - |
Capra hircus | |
| 3.4.22.1 | Mg2+ | - |
Capra hircus | |
| 3.4.22.1 | N-tosyl-lysyl chloromethylketone | - |
Capra hircus | |
| 3.4.22.1 | N-tosyl-phenylalanine chloromethylketone | - |
Capra hircus | |
| 3.4.22.1 | p-chloromercuribenzoate | - |
Capra hircus | |
| 3.4.22.1 | Zn2+ | - |
Capra hircus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.22.1 | 0.09 | 0.29 | benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide | depending on concentration of dimethylsulfoxide, 1-2.5% | Capra hircus | |
| 3.4.22.1 | 0.51 | 2.36 | benzoyl-DL-Arg-beta-naphthylamide | depending on concentration of dimethylsulfoxide, 1-2.5% | Capra hircus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.1 | lysosome | - |
Capra hircus | 5764 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 27000 | 29000 | gel filtration, SDS-PAGE | Capra hircus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Proteins + H2O | Capra hircus | turnover of intracellular proteins | ? | - |
? | |
| 3.4.22.1 | Proteins + H2O | Capra hircus | enzyme activation and conversion of precursor proteins to their active form | ? | - |
? | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 3.4.22.1 | dimethylsulfoxide | 50% of activity lost at 6% | Capra hircus |
| 3.4.22.1 | dimethylsulfoxide | inhibits | Capra hircus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | Capra hircus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.1 | side-chain modification | glycoprotein | Capra hircus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.22.1 | - |
Capra hircus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.1 | cerebrum | maximum activity in cerebrum | Capra hircus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 10 | - |
benzoyl-DL-Arg-beta-naphthylamide as substrate, in presence of 2.5% dimethylsulfoxide | Capra hircus |
| 3.4.22.1 | 40 | - |
benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide as substrate, in presence of 2.5% dimethylsulfoxide | Capra hircus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.1 | Arg-beta-naphthylamide + H2O | - |
Capra hircus | Arg + beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzoyl-DL-Arg-beta-naphthylamide + H2O | - |
Capra hircus | benzoyl-DL-Arg + beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzoyl-L-Arg-beta-naphthylamide + H2O | - |
Capra hircus | benzoyl-L-Arg + beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzoyl-L-Arg-p-nitroanilide + H2O | - |
Capra hircus | benzoyl-L-Arg + p-nitroaniline | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O | - |
Capra hircus | benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O | - |
Capra hircus | benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Arg-Arg-p-nitroanilide + H2O | - |
Capra hircus | benzyloxycarbonyl-Arg-Arg + p-nitroaniline | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Phe-Arg-4-methoxy-beta-naphthylamide + H2O | - |
Capra hircus | benzyloxycarbonyl-Phe-Arg + 4-methoxy-beta-naphthylamine | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Phe-Arg-p-nitroanilide + H2O | - |
Capra hircus | benzyloxycarbonyl-Phe-Arg + p-nitroaniline | - |
? | |
| 3.4.22.1 | benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O | - |
Capra hircus | ? + 4-methoxy-beta-naphthylamine | - |
? | |
| 3.4.22.1 | Proteins + H2O | turnover of intracellular proteins | Capra hircus | ? | - |
? | |
| 3.4.22.1 | Proteins + H2O | enzyme activation and conversion of precursor proteins to their active form | Capra hircus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 50 | - |
- |
Capra hircus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 50 | - |
fairly stable up to | Capra hircus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.22.1 | 4 | 6 | less stable below and above | Capra hircus |
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