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Literature summary extracted from

  • Perona, R.; Vallejo, C.G.
    The lysosomal proteinase of Artemia. Purification and characterization (1982), Eur. J. Biochem., 124, 357-362.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 dithiothreitol activates Artemia sp.
3.4.22.1 EDTA no effect at 0.5 mM Artemia sp.
3.4.22.1 thiol compounds required for full activity Artemia sp.

General Stability

EC Number General Stability Organism
3.4.22.1 glycerol stabilizes Artemia sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 antipain
-
 Artemia sp.
3.4.22.1 Cu2+
-
 Artemia sp.
3.4.22.1 H2O2
-
 Artemia sp.
3.4.22.1 iodoacetic acid
-
 Artemia sp.
3.4.22.1 KI
-
 Artemia sp.
3.4.22.1 L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Artemia sp.
3.4.22.1 leupeptin
-
 Artemia sp.
3.4.22.1 N-ethylmaleimide no inhibition at 1 mM Artemia sp.
3.4.22.1 p-chloromercuriphenyl sulfonic acid
-
 Artemia sp.
3.4.22.1 p-hydroxymercuribenzoate
-
 Artemia sp.
3.4.22.1 phenylmethanesulfonyl fluoride no inhibition at 10 microM Artemia sp.
3.4.22.1 Zn2+
-
 Artemia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.02
-
 protamine
-
 Artemia sp.
3.4.22.1 0.02
-
 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide
-
 Artemia sp.
3.4.22.1 0.13
-
 D-Pro-Phe-Arg-p-nitroanilide
-
 Artemia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 68000
-
 filtration, SDS-PAGE Artemia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Artemia sp.
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Artemia sp.
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.1 side-chain modification glycoprotein Artemia sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Artemia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 gastrula dormant gastrula, cysts Artemia sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Artemia sp. benzyloxycarbonyl-Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
3.4.22.1 Bovine serum albumin + H2O
-
 Artemia sp. Hydrolyzed bovine serum albumin
-
 ?
3.4.22.1 D-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Artemia sp. D-Pro-Phe-Arg + p-nitroaniline
-
 ?
3.4.22.1 D-Val-Leu-Lys-p-nitroanilide + H2O
-
 Artemia sp. D-Val-Leu-Lys + p-nitroaniline
-
 ?
3.4.22.1 Protamine + H2O
-
 Artemia sp. Hydrolyzed protamine
-
 ?
3.4.22.1 Proteins + H2O
-
 Artemia sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 monomer 1*68000, gel filtration, SDS-PAGE Artemia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 3.5
-
-
 Artemia sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 3.3 7 stable, optimum stability at pH 7.0 Artemia sp.