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Literature summary extracted from

  • Murakami, S.; Takemoto, J.; Takenaka, S.; Shinke, R.; Aoki, K.
    Purification and characterization of muconate cycloisomerase from aniline-assimilating Rhodococcus erythropolis (1998), J. Ferment. Bioeng., 85, 521-524.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.1 CuSO4
-
 Rhodococcus erythropolis
5.5.1.1 DTNB
-
 Rhodococcus erythropolis
5.5.1.1 EDTA
-
 Rhodococcus erythropolis
5.5.1.1 HgCl2
-
 Rhodococcus erythropolis
5.5.1.1 nitriloacetic acid
-
 Rhodococcus erythropolis
5.5.1.1 PCMB
-
 Rhodococcus erythropolis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.1 Co2+ is the best metal ion activator Rhodococcus erythropolis
5.5.1.1 Mn2+ activates to a lower extent than Co2+ Rhodococcus erythropolis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.1 42000
-
 x * 42000, SDS-PAGE Rhodococcus erythropolis
5.5.1.1 400000
-
 gel filtration Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.1 cis,cis-Muconate Rhodococcus erythropolis catalyzes the second step of the beta-ketoadipate pathway ?
-
 ?
5.5.1.1 cis,cis-Muconate Rhodococcus erythropolis AN-13 catalyzes the second step of the beta-ketoadipate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Rhodococcus erythropolis
-
 AN-13
-
5.5.1.1 Rhodococcus erythropolis AN-13
-
 AN-13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.1
-
 Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.1 additional information
-
-
 Rhodococcus erythropolis

Storage Stability

EC Number Storage Stability Organism
5.5.1.1 4°C, pH 7.0, enzyme retains more than 70% of the maximum activity after 24 h Rhodococcus erythropolis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.1 2-Methyl-cis,cis-muconate at 6.1% of the activity relative to cis,cis-muconate Rhodococcus erythropolis ?
-
 ?
5.5.1.1 2-Methyl-cis,cis-muconate at 6.1% of the activity relative to cis,cis-muconate Rhodococcus erythropolis AN-13 ?
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate at 5.6% of the activity relative to cis,cis-muconate Rhodococcus erythropolis beta-Methylmuconolactone
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate at 5.6% of the activity relative to cis,cis-muconate Rhodococcus erythropolis AN-13 beta-Methylmuconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Rhodococcus erythropolis (+)-Muconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Rhodococcus erythropolis AN-13 (+)-Muconolactone
-
 ?
5.5.1.1 cis,cis-Muconate catalyzes the second step of the beta-ketoadipate pathway Rhodococcus erythropolis ?
-
 ?
5.5.1.1 cis,cis-Muconate catalyzes the second step of the beta-ketoadipate pathway Rhodococcus erythropolis AN-13 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.1 ? x * 42000, SDS-PAGE Rhodococcus erythropolis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.5.1.1 60
-
 10 min, less than 20% loss of activity Rhodococcus erythropolis
5.5.1.1 65
-
 10 min, enzyme loses most of its activity Rhodococcus erythropolis