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Literature summary extracted from

  • Tsakalidou, E.; Anastasiou, R.; Papadimitriou, K.; Manopoulou, E.; Kalantzopoulos, G.
    Purification and characterisation of an intracellular X-prolyl-dipeptidyl aminopeptidase from Streptococus thermophilus ACA-DC 4 (1997), J. Biotechnol., 59, 203-211.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 EDTA
-
 Streptococcus thermophilus
3.4.14.5 iodoacetate
-
 Streptococcus thermophilus
3.4.14.5 phenylmethylsulfonyl fluoride
-
 Streptococcus thermophilus
3.4.14.11 EDTA 5 mM, 40% inhibition Streptococcus thermophilus
3.4.14.11 iodoacetate 5 mM, 64% inhibition Streptococcus thermophilus
3.4.14.11 phenylmethylsulfonyl fluoride 5 mM, complete inhibition Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 3.1
-
 Gly-Pro-p-nitroanilide
-
 Streptococcus thermophilus
3.4.14.11 3.1
-
 Gly-Pro-4-nitroanilide pH 7.0, 50°C Streptococcus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.11 intracellular
-
 Streptococcus thermophilus 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 80000
-
 2 * 80000, SDS-PAGE Streptococcus thermophilus
3.4.14.5 160000
-
 gel filtration Streptococcus thermophilus
3.4.14.11 80000
-
 2 * 80000, SDS-PAGE Streptococcus thermophilus
3.4.14.11 160000
-
 gel filtration Streptococcus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Streptococcus thermophilus
-
 ACA-DC 4
-
3.4.14.5 Streptococcus thermophilus ACA-DC 4
-
 ACA-DC 4
-
3.4.14.11 Streptococcus thermophilus
-
 ACA-DC 4
-
3.4.14.11 Streptococcus thermophilus ACA-DC 4
-
 ACA-DC 4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Streptococcus thermophilus
3.4.14.11
-
 Streptococcus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 285
-
-
 Streptococcus thermophilus
3.4.14.11 285
-
-
 Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-4-nitroanilide + H2O 1% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ala + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-Gly + H2O 72% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ala-Pro + Gly
-
 ?
3.4.14.5 Arg-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Arg + 4-nitroaniline
-
 ?
3.4.14.5 Arg-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Arg + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O 55% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O 55% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Phe-4-nitroanilide + H2O 1% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Gly-Phe + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus thermophilus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus thermophilus ACA-DC 4 Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-Arg-Pro + H2O as active as with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?
3.4.14.5 Gly-Pro-Arg-Pro + H2O as active as with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 ?
-
 ?
3.4.14.5 Ile-Pro-Ile + H2O 34% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?
3.4.14.5 Phe-4-nitroanilide + H2O 7% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Phe + 4-nitroaniline
-
 ?
3.4.14.5 Phe-4-nitroanilide + H2O 7% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ACA-DC 4 Phe + 4-nitroaniline
-
 ?
3.4.14.5 Pro-4-nitroanilide + H2O 2% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Pro + 4-nitroaniline
-
 ?
3.4.14.5 Val-Pro-Leu + H2O 83% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus ?
-
 ?
3.4.14.11 Ala-Pro-Gly + H2O 72% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ala-Pro + Gly
-
 ?
3.4.14.11 Arg-Pro 4-nitroanilide + H2O 55% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Gly-Pro 4-nitroanilide + H2O
-
 Streptococcus thermophilus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.11 Gly-Pro-Arg-Pro + H2O as active as Gly-Pro-4-nitroanilide Streptococcus thermophilus Gly-Pro + Arg-Pro
-
 ?
3.4.14.11 Ile-Pro-Ile + H2O 34% of the activity with Gly-Pro-4-nitroanilide Streptococcus thermophilus Ile-Pro + Ile
-
 ?
3.4.14.11 Val-Pro-Leu + H2O 83% of the activity with Gly-Pro-nitroanilide Streptococcus thermophilus Val-Pro + Leu
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 dimer 2 * 80000, SDS-PAGE Streptococcus thermophilus
3.4.14.11 dimer 2 * 80000, SDS-PAGE Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 50
-
-
 Streptococcus thermophilus
3.4.14.11 50
-
 hydrolysis of Gly-Pro-4-nitroanilide Streptococcus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.14.5 10 60 10°C: 17% of maximal activity, about about 40% of maximal activity at 25°C and at 60°C Streptococcus thermophilus
3.4.14.11 40 60 40°C: about 15% of maximal activity, 60°C: about 40% of maximal activity Streptococcus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 50
-
 10 min, stable Streptococcus thermophilus
3.4.14.5 60
-
 1 min, in absence of substrate, 96% loss of activity Streptococcus thermophilus
3.4.14.5 70
-
 1 min, complete loss of activity Streptococcus thermophilus
3.4.14.11 60
-
 1 min in absence of substrate, 96% loss of activity Streptococcus thermophilus
3.4.14.11 70
-
 1 min, complete loss of activity Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7
-
-
 Streptococcus thermophilus
3.4.14.11 7
-
 hydrolysis of Gly-Pro-4-nitroanilide Streptococcus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5 9 pH 5.0: about 15% of maximal activity, pH 9.0: about 85% of maximal activity Streptococcus thermophilus
3.4.14.11 6 9 pH 6.0: about 90% of maximal activity, pH 9.0: about 85% of maximal activity, hydrolysis of Gly-Pro-4-nitroanilide Streptococcus thermophilus