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Literature summary extracted from
- Aminopeptidase P from rat brain. Purification and action on bioactive peptides (1991), Eur. J. Biochem., 198, 451-458.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | 1,10-phenanthroline | - |
Rattus norvegicus |  |
| 3.4.11.9 | 4-hydroxymercuribenzoate | - |
Rattus norvegicus | |
| 3.4.11.9 | amastatin | - |
Rattus norvegicus | |
| 3.4.11.9 | Aprotinin | - |
Rattus norvegicus | |
| 3.4.11.9 | bestatin | - |
Rattus norvegicus | |
| 3.4.11.9 | Ca2+ | 1 mM CaCl2, 27% inhibition | Rattus norvegicus | |
| 3.4.11.9 | Cd2+ | - |
Rattus norvegicus | |
| 3.4.11.9 | Co2+ | 1 mM CoCl2, 90% inhibition | Rattus norvegicus | |
| 3.4.11.9 | EDTA | - |
Rattus norvegicus | |
| 3.4.11.9 | EGTA | - |
Rattus norvegicus | |
| 3.4.11.9 | Hg2+ | - |
Rattus norvegicus | |
| 3.4.11.9 | iodoacetate | - |
Rattus norvegicus | |
| 3.4.11.9 | NEM | - |
Rattus norvegicus | |
| 3.4.11.9 | Pb2+ | - |
Rattus norvegicus | |
| 3.4.11.9 | puromycin | - |
Rattus norvegicus | |
| 3.4.11.9 | Zn2+ | 1 mM ZnSO4, 96% inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 0.16 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.28 | - |
bradykinin | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.6 | - |
Tyr-Pro-Phe-Pro-Gly-Pro-Ile | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.72 | - |
Tyr-Pro-Leu-Gly-NH2 | - |
Rattus norvegicus | |
| 3.4.11.9 | 1.02 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
| 3.4.11.9 | 1.23 | - |
Arg-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 1.33 | - |
L-Tyr-L-Pro-L-Phe-NH2 | - |
Rattus norvegicus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.9 | cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 143000 | - |
with 0.14 M NaCl, gel filtration | Rattus norvegicus |
| 3.4.11.9 | 218000 | - |
without NaCl, gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.9 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | brain | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 31.7 | - |
- |
Rattus norvegicus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.11.9 | 4°C, bicine buffer, pH 7.8, 1 mM dithiothreitol, 1 mM MnCl2, stable for several weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.9 | Ala-Pro + H2O | - |
Rattus norvegicus | Ala + Pro | - |
? | |
| 3.4.11.9 | Arg-Pro + H2O | - |
Rattus norvegicus | Arg + Pro | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro + H2O | - |
Rattus norvegicus | Arg + Pro-Pro | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Rattus norvegicus | Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
? | |
| 3.4.11.9 | L-Tyr-L-Pro-L-Phe-NH2 + H2O | - |
Rattus norvegicus | L-Tyr + L-Pro-L-Phe-NH2 | - |
? | |
| 3.4.11.9 | additional information | the enzyme liberates all unblocked preferentially basic or hydrophobic ultimate amino acids from dipeptides, tripeptides and oligopeptides with N-terminal Xaa-Pro- sequences, overview | Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Tyr-Pro-Leu-Gly-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Tyr-Pro-Phe-Pro-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O | - |
Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 37 | - |
pH 7.8, graduall loss of activity | Rattus norvegicus |
| 3.4.11.9 | 45 | - |
pH 7.8, rapid denaturation above | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 26 | - |
Tyr-Pro-Phe-Pro-Gly-Pro-Ile | - |
Rattus norvegicus | |
| 3.4.11.9 | 32 | - |
Tyr-Pro-Phe-Pro-Gly | - |
Rattus norvegicus | |
| 3.4.11.9 | 38 | - |
bradykinin | - |
Rattus norvegicus | |
| 3.4.11.9 | 40 | - |
Tyr-Pro-Leu-Gly-NH2 | - |
Rattus norvegicus | |
| 3.4.11.9 | 49 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 58 | - |
L-Tyr-L-Pro-L-Phe-NH2 | - |
Rattus norvegicus | |
| 3.4.11.9 | 73 | - |
Arg-Pro | - |
Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 7.6 | 8 | hydrolysis of Arg-Pro-Pro, Ala-Pro or Tyr-Pro-Leu-Gly-NH2 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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