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Literature summary extracted from
- Nicotinic acid metabolism. VI. Purification and properties of alpha-methyleneglutarate mutase (B12-dependent) and methylitaconate isomerase (1971), J. Biol. Chem., 246, 3378-3388.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.6 | EDTA | 10 mM, 30% increase in activity | Eubacterium barkeri |  |
| 5.3.3.6 | mercaptan | required for optimal activity | Eubacterium barkeri | |
| 5.3.3.6 | additional information | enzyme contains sulfhydryl groups | Eubacterium barkeri | |
| 5.4.99.4 | sulfhydryl groups | the enzyme is a sulfhydryl protein | Eubacterium barkeri | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.99.4 | enzyme rapidly decays, when stored at low protein concentrations, 1 mg/ml. Complete loss of activity after 3 days at 0°C and 38% loss of activity after 6 days at -80°C | Eubacterium barkeri |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.6 | GSSG | - |
Eubacterium barkeri | |
| 5.3.3.6 | iodoacetic acid | - |
Eubacterium barkeri | |
| 5.3.3.6 | p-chloromercuriphenyl sulfonic acid | - |
Eubacterium barkeri | |
| 5.4.99.4 | 1-Methyl-1,2-cis-cyclopropanedicarboxylate | - |
Eubacterium barkeri | |
| 5.4.99.4 | 1-Methyl-1,2-trans-cyclopropanedicarboxylate | - |
Eubacterium barkeri | |
| 5.4.99.4 | glutaconate | - |
Eubacterium barkeri | |
| 5.4.99.4 | iodoacetamide | alkylated enzyme | Eubacterium barkeri | |
| 5.4.99.4 | Itaconate | - |
Eubacterium barkeri | |
| 5.4.99.4 | mesaconate | - |
Eubacterium barkeri | |
| 5.4.99.4 | p-chloromercuriphenylsulfonic acid | - |
Eubacterium barkeri | |
| 5.4.99.4 | succinate | - |
Eubacterium barkeri | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.3.6 | 4 | - |
dimethylmaleate | - |
Eubacterium barkeri | |
| 5.3.3.6 | 6.2 | - |
methylitaconate | - |
Eubacterium barkeri | |
| 5.4.99.4 | 7.1 | - |
2-Methyleneglutarate | - |
Eubacterium barkeri | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.3.6 | additional information | no metal ion required | Eubacterium barkeri |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.6 | 140000 | - |
sucrose density gradient centrifugation | Eubacterium barkeri |
| 5.4.99.4 | 170000 | - |
sucrose density gradient centrifugation | Eubacterium barkeri |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.4 | additional information | Eubacterium barkeri | enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.3.6 | Eubacterium barkeri | - |
- |
- |
| 5.4.99.4 | Eubacterium barkeri | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.3.6 | - |
Eubacterium barkeri |
| 5.4.99.4 | - |
Eubacterium barkeri |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.6 | additional information | - |
- |
Eubacterium barkeri |
| 5.4.99.4 | additional information | - |
- |
Eubacterium barkeri |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.3.3.6 | -20°C, protein concentration 1 mg/ml, little loss of activity after 60 days | Eubacterium barkeri |
| 5.3.3.6 | -80°C, protein concentration 10 mg/ml, less than 10% loss of activity after 6 months | Eubacterium barkeri |
| 5.3.3.6 | 0°C, protein concentration 1 mg/ml, little loss of activity after 6 days | Eubacterium barkeri |
| 5.4.99.4 | -80°C, protein concentration 1 mg/ml, 38% loss of activity after 6 days | Eubacterium barkeri |
| 5.4.99.4 | 0°C, protein concentration 1 mg/ml, complete loss of activity after 3 days | Eubacterium barkeri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.3.6 | Methylitaconate | r | Eubacterium barkeri | Dimethylmaleate | - |
? | |
| 5.4.99.4 | 2-Methyleneglutarate | r | Eubacterium barkeri | 2-Methylene-3-methylsuccinate | i.e methylitaconate | ? | |
| 5.4.99.4 | 2-Methyleneglutarate | the equilibrium favours the formation of 2-methyleneglutarate | Eubacterium barkeri | 2-Methylene-3-methylsuccinate | i.e methylitaconate | ? | |
| 5.4.99.4 | additional information | enzyme is induced by growth on nicotinic acid and is not detectable in cells grown on glucose | Eubacterium barkeri | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.3.6 | 6.8 | 8.2 | - |
Eubacterium barkeri |
| 5.4.99.4 | 7.5 | 8 | sharp decrease in activity below pH 7.5 and above pH 8 | Eubacterium barkeri |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.4 | cobamide | required | Eubacterium barkeri | |
| 5.4.99.4 | cobamide | e.g. alpha-(5,6-dimethylbenzimidazolyl)cobamide coenzyme, Km: 0.000073 mM, alpha-(benzimidazolyl)-cobamide coenzyme, Km: 0.0003 mM, or alpha-(adenyl)-cobamide coenzyme, Km: 0.00125 mM. The most effective coenzyme is alpha(5,6-dimethylbenzimidazolyl)-cobamide coenzyme | Eubacterium barkeri | |
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