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Literature summary extracted from

  • Bosmann, H.B.
    Membrane marker enzymes. Characterization of an arylesterase of guinea pig cerebral cortex utilizing p-nitrophenyl acetate as substrate (1972), Biochim. Biophys. Acta, 276, 180-191.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 Ca2+
-
 Cavia porcellus
3.1.1.2 Co2+
-
 Cavia porcellus
3.1.1.2 Cu2+
-
 Cavia porcellus
3.1.1.2 Fe2+
-
 Cavia porcellus
3.1.1.2 Hg2+
-
 Cavia porcellus
3.1.1.2 Mg2+ not Cavia porcellus
3.1.1.2 additional information not: Cd2+ Cavia porcellus
3.1.1.2 p-hydroxymercuribenzoate
-
 Cavia porcellus
3.1.1.2 Pb2+
-
 Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.000043
-
 4-nitrophenyl acetate 37°C, pH 7.6 Cavia porcellus
3.1.1.2 0.043
-
 p-nitrophenyl acetate
-
 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.2 microsome
-
 Cavia porcellus
-
-
3.1.1.2 microsome good marker enzyme Cavia porcellus
-
-
3.1.1.2 rough endoplasmic reticulum good marker enzyme Cavia porcellus 5791
-
3.1.1.2 synaptosome
-
 Cavia porcellus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 Cd2+ slightly activating Cavia porcellus
3.1.1.2 Cd2+ can partially replace Mn2+ in activation Cavia porcellus
3.1.1.2 Mg2+ slightly acitvating Cavia porcellus
3.1.1.2 Mn2+ MnCl2 required Cavia porcellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.2 78000
-
 and 180000, whether the former is a subunit of the latter is not known, gel filtration Cavia porcellus
3.1.1.2 180000
-
 and 78000, whether the latter is a subunit of the former is not known, gel filtration Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.2 phenyl acetate + H2O Cavia porcellus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2
-
 Cavia porcellus
3.1.1.2 2 forms, pI 4.9 form and pI 5.9 form Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 cerebral cortex
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.2 155
-
-
 Cavia porcellus
3.1.1.2 155.6
-
 37°C, pH 7.6 Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Cavia porcellus 4-nitrophenol + acetate
-
 ?
3.1.1.2 4-nitrophenyl acetate + H2O
-
 Cavia porcellus 4-nitrophenol + acetate
-
 ir
3.1.1.2 phenyl acetate + H2O
-
 Cavia porcellus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.2 37
-
 assay at Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.2 100
-
 boiling completely abolishes activity Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.2 7.6
-
-
 Cavia porcellus
3.1.1.2 7.6
-
 p-nitrophenyl acetate Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.2 7 9 p-nitrophenyl acetate Cavia porcellus
3.1.1.2 7.3 8.2 about 50% of activity maximum at pH 7.3 and 8.2 Cavia porcellus