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Literature summary extracted from
- Purification and properties of methylmalonyl coenzyme A mutase from human liver (1982), Arch. Biochem. Biophys., 214, 815-823.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.4.99.2 | repeated freezing and thawing results in a gradual loss of activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | (R)-2-methyl-3-oxopropanoyl-CoA | substrate inhibition | Homo sapiens |  |
| 5.4.99.2 | Ca2+ | at high concentrations | Homo sapiens | |
| 5.4.99.2 | Mg2+ | at high concentrations | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.2 | 1.5 | - |
(R)-2-methyl-3-oxopropanoyl-CoA | - |
Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | Cl- | stimulates | Homo sapiens | |
| 5.4.99.2 | PO43- | required, maximal activity at 50 mM | Homo sapiens | |
| 5.4.99.2 | SO42- | stimulates | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 77500 | - |
2 * 77500, SDS-PAGE | Homo sapiens |
| 5.4.99.2 | 150000 | - |
gel filtration | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.2 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.99.2 | liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 14 | - |
- |
Homo sapiens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.4.99.2 | -20°C, 1.5 mg protein /ml in 50 mM potassium phosphate, pH 7.4, stable for at least 6 months | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.2 | (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Homo sapiens | ? | - |
? | |
| 5.4.99.2 | (R)-2-methylmalonyl-CoA | - |
Homo sapiens | succinyl-CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.2 | dimer | 2 * 77500, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 50 | - |
15 min, stable below | Homo sapiens |
| 5.4.99.2 | 55 | - |
t1/2: 3 min | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.2 | 6.5 | 8 | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.2 | cobamide | one molecule of enzyme contains about 2 molecules of adenosylcobalamin | Homo sapiens | |
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Release 2023.1 (January 2023)
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